research project topics in environmental biotechnology

200+ Biotechnology Research Topics: Let’s Shape the Future

In the dynamic landscape of scientific exploration, biotechnology stands at the forefront, revolutionizing the way we approach healthcare, agriculture, and environmental sustainability. This interdisciplinary field encompasses a vast array of research topics that hold the potential to reshape our world. 

In this blog post, we will delve into the realm of biotechnology research topics, understanding their significance and exploring the diverse avenues that researchers are actively investigating.

Overview of Biotechnology Research

Table of Contents

Biotechnology, at its core, involves the application of biological systems, organisms, or derivatives to develop technologies and products for the benefit of humanity. 

The scope of biotechnology research is broad, covering areas such as genetic engineering, biomedical engineering, environmental biotechnology, and industrial biotechnology. Its interdisciplinary nature makes it a melting pot of ideas and innovations, pushing the boundaries of what is possible.

How to Select The Best Biotechnology Research Topics?

• Identify Your Interests

Start by reflecting on your own interests within the broad field of biotechnology. What aspects of biotechnology excite you the most? Identifying your passion will make the research process more engaging.

• Stay Informed About Current Trends

Keep up with the latest developments and trends in biotechnology. Subscribe to scientific journals, attend conferences, and follow reputable websites to stay informed about cutting-edge research. This will help you identify gaps in knowledge or areas where advancements are needed.

• Consider Societal Impact

Evaluate the potential societal impact of your chosen research topic. How does it contribute to solving real-world problems? Biotechnology has applications in healthcare, agriculture, environmental conservation, and more. Choose a topic that aligns with the broader goal of improving quality of life or addressing global challenges.

• Assess Feasibility and Resources

Evaluate the feasibility of your research topic. Consider the availability of resources, including laboratory equipment, funding, and expertise. A well-defined and achievable research plan will increase the likelihood of successful outcomes.

• Explore Innovation Opportunities

Look for opportunities to contribute to innovation within the field. Consider topics that push the boundaries of current knowledge, introduce novel methodologies, or explore interdisciplinary approaches. Innovation often leads to groundbreaking discoveries.

• Consult with Mentors and Peers

Seek guidance from mentors, professors, or colleagues who have expertise in biotechnology. Discuss your research interests with them and gather insights. They can provide valuable advice on the feasibility and significance of your chosen topic.

• Balance Specificity and Breadth

Strike a balance between biotechnology research topics that are specific enough to address a particular aspect of biotechnology and broad enough to allow for meaningful research. A topic that is too narrow may limit your research scope, while one that is too broad may lack focus.

• Consider Ethical Implications

Be mindful of the ethical implications of your research. Biotechnology, especially areas like genetic engineering, can raise ethical concerns. Ensure that your chosen topic aligns with ethical standards and consider how your research may impact society.

• Evaluate Industry Relevance

Consider the relevance of your research topic to the biotechnology industry. Industry-relevant research has the potential for practical applications and may attract funding and collaboration opportunities.

• Stay Flexible and Open-Minded

Be open to refining or adjusting your research topic as you delve deeper into the literature and gather more information. Flexibility is key to adapting to new insights and developments in the field.

200+ Biotechnology Research Topics: Category-Wise

Genetic engineering.

• CRISPR-Cas9: Recent Advances and Applications
• Gene Editing for Therapeutic Purposes: Opportunities and Challenges
• Precision Medicine and Personalized Genomic Therapies
• Genome Sequencing Technologies: Current State and Future Prospects
• Synthetic Biology: Engineering New Life Forms
• Genetic Modification of Crops for Improved Yield and Resistance
• Ethical Considerations in Human Genetic Engineering
• Gene Therapy for Neurological Disorders
• Epigenetics: Understanding the Role of Gene Regulation
• CRISPR in Agriculture: Enhancing Crop Traits

Biomedical Engineering

• Tissue Engineering: Creating Organs in the Lab
• 3D Printing in Biomedical Applications
• Advances in Drug Delivery Systems
• Nanotechnology in Medicine: Theranostic Approaches
• Bioinformatics and Computational Biology in Biomedicine
• Wearable Biomedical Devices for Health Monitoring
• Stem Cell Research and Regenerative Medicine
• Precision Oncology: Tailoring Cancer Treatments
• Biomaterials for Biomedical Applications
• Biomechanics in Biomedical Engineering

Environmental Biotechnology

• Bioremediation of Polluted Environments
• Waste-to-Energy Technologies: Turning Trash into Power
• Sustainable Agriculture Practices Using Biotechnology
• Bioaugmentation in Wastewater Treatment
• Microbial Fuel Cells: Harnessing Microorganisms for Energy
• Biotechnology in Conservation Biology
• Phytoremediation: Plants as Environmental Cleanup Agents
• Aquaponics: Integration of Aquaculture and Hydroponics
• Biodiversity Monitoring Using DNA Barcoding
• Algal Biofuels: A Sustainable Energy Source

Industrial Biotechnology

• Enzyme Engineering for Industrial Applications
• Bioprocessing and Bio-manufacturing Innovations
• Industrial Applications of Microbial Biotechnology
• Bio-based Materials: Eco-friendly Alternatives
• Synthetic Biology for Industrial Processes
• Metabolic Engineering for Chemical Production
• Industrial Fermentation: Optimization and Scale-up
• Biocatalysis in Pharmaceutical Industry
• Advanced Bioprocess Monitoring and Control
• Green Chemistry: Sustainable Practices in Industry

Emerging Trends in Biotechnology

• CRISPR-Based Diagnostics: A New Era in Disease Detection
• Neurobiotechnology: Advancements in Brain-Computer Interfaces
• Advances in Nanotechnology for Healthcare
• Computational Biology: Modeling Biological Systems
• Organoids: Miniature Organs for Drug Testing
• Genome Editing in Non-Human Organisms
• Biotechnology and the Internet of Things (IoT)
• Exosome-based Therapeutics: Potential Applications
• Biohybrid Systems: Integrating Living and Artificial Components
• Metagenomics: Exploring Microbial Communities

Ethical and Social Implications

• Ethical Considerations in CRISPR-Based Gene Editing
• Privacy Concerns in Personal Genomic Data Sharing
• Biotechnology and Social Equity: Bridging the Gap
• Dual-Use Dilemmas in Biotechnological Research
• Informed Consent in Genetic Testing and Research
• Accessibility of Biotechnological Therapies: Global Perspectives
• Human Enhancement Technologies: Ethical Perspectives
• Biotechnology and Cultural Perspectives on Genetic Modification
• Social Impact Assessment of Biotechnological Interventions
• Intellectual Property Rights in Biotechnology

Computational Biology and Bioinformatics

• Machine Learning in Biomedical Data Analysis
• Network Biology: Understanding Biological Systems
• Structural Bioinformatics: Predicting Protein Structures
• Data Mining in Genomics and Proteomics
• Systems Biology Approaches in Biotechnology
• Comparative Genomics: Evolutionary Insights
• Bioinformatics Tools for Drug Discovery
• Cloud Computing in Biomedical Research
• Artificial Intelligence in Diagnostics and Treatment
• Computational Approaches to Vaccine Design

Health and Medicine

• Vaccines and Immunotherapy: Advancements in Disease Prevention
• CRISPR-Based Therapies for Genetic Disorders
• Infectious Disease Diagnostics Using Biotechnology
• Telemedicine and Biotechnology Integration
• Biotechnology in Rare Disease Research
• Gut Microbiome and Human Health
• Precision Nutrition: Personalized Diets Using Biotechnology
• Biotechnology Approaches to Combat Antibiotic Resistance
• Point-of-Care Diagnostics for Global Health
• Biotechnology in Aging Research and Longevity

Agricultural Biotechnology

• CRISPR and Gene Editing in Crop Improvement
• Precision Agriculture: Integrating Technology for Crop Management
• Biotechnology Solutions for Food Security
• RNA Interference in Pest Control
• Vertical Farming and Biotechnology
• Plant-Microbe Interactions for Sustainable Agriculture
• Biofortification: Enhancing Nutritional Content in Crops
• Smart Farming Technologies and Biotechnology
• Precision Livestock Farming Using Biotechnological Tools
• Drought-Tolerant Crops: Biotechnological Approaches

Biotechnology and Education

• Integrating Biotechnology into STEM Education
• Virtual Labs in Biotechnology Teaching
• Biotechnology Outreach Programs for Schools
• Online Courses in Biotechnology: Accessibility and Quality
• Hands-on Biotechnology Experiments for Students
• Bioethics Education in Biotechnology Programs
• Role of Internships in Biotechnology Education
• Collaborative Learning in Biotechnology Classrooms
• Biotechnology Education for Non-Science Majors
• Addressing Gender Disparities in Biotechnology Education

Funding and Policy

• Government Funding Initiatives for Biotechnology Research
• Private Sector Investment in Biotechnology Ventures
• Impact of Intellectual Property Policies on Biotechnology
• Ethical Guidelines for Biotechnological Research
• Public-Private Partnerships in Biotechnology
• Regulatory Frameworks for Gene Editing Technologies
• Biotechnology and Global Health Policy
• Biotechnology Diplomacy: International Collaboration
• Funding Challenges in Biotechnology Startups
• Role of Nonprofit Organizations in Biotechnological Research

Biotechnology and the Environment

• Biotechnology for Air Pollution Control
• Microbial Sensors for Environmental Monitoring
• Remote Sensing in Environmental Biotechnology
• Climate Change Mitigation Using Biotechnology
• Circular Economy and Biotechnological Innovations
• Marine Biotechnology for Ocean Conservation
• Bio-inspired Design for Environmental Solutions
• Ecological Restoration Using Biotechnological Approaches
• Impact of Biotechnology on Biodiversity
• Biotechnology and Sustainable Urban Development

Biosecurity and Biosafety

• Biosecurity Measures in Biotechnology Laboratories
• Dual-Use Research and Ethical Considerations
• Global Collaboration for Biosafety in Biotechnology
• Security Risks in Gene Editing Technologies
• Surveillance Technologies in Biotechnological Research
• Biosecurity Education for Biotechnology Professionals
• Risk Assessment in Biotechnology Research
• Bioethics in Biodefense Research
• Biotechnology and National Security
• Public Awareness and Biosecurity in Biotechnology

Industry Applications

• Biotechnology in the Pharmaceutical Industry
• Bioprocessing Innovations for Drug Production
• Industrial Enzymes and Their Applications
• Biotechnology in Food and Beverage Production
• Applications of Synthetic Biology in Industry
• Biotechnology in Textile Manufacturing
• Cosmetic and Personal Care Biotechnology
• Biotechnological Approaches in Renewable Energy
• Advanced Materials Production Using Biotechnology
• Biotechnology in the Automotive Industry

Miscellaneous Topics

• DNA Barcoding in Species Identification
• Bioart: The Intersection of Biology and Art
• Biotechnology in Forensic Science
• Using Biotechnology to Preserve Cultural Heritage
• Biohacking: DIY Biology and Citizen Science
• Microbiome Engineering for Human Health
• Environmental DNA (eDNA) for Biodiversity Monitoring
• Biotechnology and Astrobiology: Searching for Life Beyond Earth
• Biotechnology and Sports Science
• Biotechnology and the Future of Space Exploration

Challenges and Ethical Considerations in Biotechnology Research

As biotechnology continues to advance, it brings forth a set of challenges and ethical considerations. Biosecurity concerns, especially in the context of gene editing technologies, raise questions about the responsible use of powerful tools like CRISPR. 

Ethical implications of genetic manipulation, such as the creation of designer babies, demand careful consideration and international collaboration to establish guidelines and regulations. 

Moreover, the environmental and social impact of biotechnological interventions must be thoroughly assessed to ensure responsible and sustainable practices.

Funding and Resources for Biotechnology Research

The pursuit of biotechnology research topics requires substantial funding and resources. Government grants and funding agencies play a pivotal role in supporting research initiatives. 

Simultaneously, the private sector, including biotechnology companies and venture capitalists, invest in promising projects. Collaboration and partnerships between academia, industry, and nonprofit organizations further amplify the impact of biotechnological research.

Future Prospects of Biotechnology Research

As we look to the future, the integration of biotechnology with other scientific disciplines holds immense potential. Collaborations with fields like artificial intelligence, materials science, and robotics may lead to unprecedented breakthroughs. 

The development of innovative technologies and their application to global health and sustainability challenges will likely shape the future of biotechnology.

In conclusion, biotechnology research is a dynamic and transformative force with the potential to revolutionize multiple facets of our lives. The exploration of diverse biotechnology research topics, from genetic engineering to emerging trends like synthetic biology and nanobiotechnology, highlights the breadth of possibilities within this field. 

However, researchers must navigate challenges and ethical considerations to ensure that biotechnological advancements are used responsibly for the betterment of society. 

With continued funding, collaboration, and a commitment to ethical practices, the future of biotechnology research holds exciting promise, propelling us towards a more sustainable and technologically advanced world.

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Biotechnology for the Environment is an open access, peer-reviewed journal featuring studies that explore the advancements and applications of biotechnology for environmental management, protection, and sustainability, including agriculture and forestry. The journal also aims to advance the discussion around the complex interplay between environmental issues and bioproduction through insightful and diverse research.

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• v.2012; 2012

Biotechnological Tools for Environmental Sustainability: Prospects and Challenges for Environments in Nigeria—A Standard Review

Chukwuma s. ezeonu.

1 Industrial Biochemistry and Environmental Biotechnology Unit, Chemical Sciences Department, Godfrey Okoye University, P.M.B. 01014, Enugu, Nigeria

Richard Tagbo

Ephraim n. anike.

2 Pure and Industrial Chemistry Unit, Chemical Sciences Department, Godfrey Okoye University, P.M.B. 01014, Enugu, Nigeria

Obinna A. Oje

3 Pollution Control and Biotechnology Unit, Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria

Ikechukwu N. E. Onwurah

The environment is a very important component necessary for the existence of both man and other biotic organisms. The degree of sustainability of the physical environment is an index of the survival and well-being of the entire components in it. Additionally, it is not sufficient to try disposing toxic/deleterious substances with any known method. The best method of sustaining the environment is such that returns back all the components (wastes) in a recyclable way so that the waste becomes useful and helps the biotic and abiotic relationship to maintain an aesthetic and healthy equilibrium that characterizes an ideal environment. In this study, the method investigated includes biological method of environmental sustainability which seeks to investigate the various biotechnological tools (biotools) in current use and those undergoing investigations for future use.

1. Introduction

Biotechnological tools are those processes of bioscientific interests that use the chemistry of living organisms through cell manipulation to develop new and alternative methods aimed at cleaner and more effective ways of producing traditional products and at the same time maintain the natural and aesthetic beauty of the environment. Biotechnology is the current trend in production processes across the world, as opposed to the conventional chemical synthesis of products. The reason is due to the fact that biotechnological methods are ecofriendly while the latter method adds pollutants and waste into our environment. A lot of problems associated with conventional methods of pollutant treatment by incineration or landfills have given the impetus on the need for alternative, economical, and reliable biological methods of pollution treatments.

Chen et al. [ 1 ] enumerated vividly that environmental biotechnology refers to the utilization of microorganisms to improve environmental quality. Although the field of environmental biotechnology has been around for decades, starting with the activated sludge and anaerobic digestion in the early 20th century, the introduction of new technologies from modern microbiology and molecular biology has enabled engineers and scientists to tackle the more contemporary environment problems such as detoxification of hazardous wastes through the use of living organisms.

As the earth's human population has increased, natural ecosystems have declined and changes in the balance of natural cycles have had a negative impact on both humans and other living systems. Thus, there is abundant scientific evidence that humanity is living unsustainably, and returning human use of natural resources to within limits will require a major collective effort [ 2 ]. Given the challenges of population increase and its attendant problems of pollution increase, biotechnology remains the most reliable means of environmental sustenance. The world is currently endangered; government and people of many counties are concerned about this endemicity of pollutants (most of which are recalcitrant) in our otherwise aesthetic environment. Africa generally and Nigeria in particular have not imbibed maximally the benefit of using biotechnology in maintenance of the beautiful environment. This paper will address the issues relating to the use of biotechnological methods vis-à-vis biotools in solving the problems of environmental degradation, with a view to encourage the adoption of these biotechnological methods in Nigeria, Africa, and other countries where waste has been a menace to the environments.

2. Environmental Sustainability

Sustainability is the capacity to endure. The word sustainability is derived from the Latin sustinere (tenere, to hold; sus, up). In ecology the word describes how biological systems remain diverse and productive over times. For humans it is the potential for long-term maintenance of well-being, which in turn depends on the well-being of the natural world and the responsible use of natural resources ( http://en.wikipedia.org/wiki/Environmental_Sustainability_Index ) [ 3 ]. Environmental sustainability is the process of making sure current processes of interaction with the environment are pursued with the idea of keeping the environment as pristine as naturally possible based on ideal-seeking behaviours. An “unsustainable situation” occurs when natural capital (the sum total of nature's resources) is used up faster than it can be replenished. Sustainability requires that human activity only uses nature's resources at a rate at which they can be replenished naturally. Theoretically, the long-term result of environmental degradation is the inability to sustain human life. Such degradation on a global scale could imply extinction for humanity [ 4 ].

A healthy environment is one that provides vital goods and services to humans as well as other organisms within its ecosystem. This can be achieved in two ways and include discovering ways of reducing negative human impact and enhancing the well-being and vitality of all living organisms (plants and animals) in the environment. Daly [ 5 ] suggested three broad criteria for ecological sustainability: renewable resources should provide a sustainable yield (the rate of harvest should not exceed the rate of regeneration); for nonrenewable resources there should be equivalent development of renewable substitutes; waste generation should not exceed the assimilative capacity of the environment.

It is important to also clearly define what the environment is to the humans who are the focus and are adversely affected positively or negatively according to their activities within their surroundings. Thus, Bankole [ 6 ] reported that “Environment” refers to the physical surroundings of man, of which he is part and on which he depends for his activities, like physiological functioning, production, and consumption. His physical environment stretches from air, water, and land to natural resources like metals, energy carriers, soil, and plants, animals, and ecosystems. For urbanized man, a large part of his environment is man-made. But even then, the artificial environments (buildings, roads) and implements (clothes, automobiles) are the result of an input of both labour and natural resources.

3. Environmental Sustainability Index (ESI)

This is a composite index tracking 21 elements of environment sustainability covering natural resource endowments, past and present pollution levels, environmental management efforts, contributions to protection of the global commons, and a society's capacity to improve its environmental performance over time [ 7 ].

The Environmental Sustainability Index was developed and published between 1999 and 2005 by Yale University's Centre for Environmental Law and Policy in collaboration with Columbia University's Centre for International Earth Science Information Network (CIESIN), and the World Economic Forum. The ESI developed to evaluate environmental sustainability relative to the paths of other countries. Due to a shift in focus by the terms developing the ESI, a new index was developed, the Environmental Performance Index (EPI) that uses the outcome-oriented indicators, then works as a benchmark index that can be more easily used by policy makers, environmental scientists, advocates and the general public [ 8 ].

4. The Nigerian Physical Environment

4.1. the niger delta environment.

Nigeria has one of the worst environmental records in the world. In late 1995, Nigeria's execution of eight environmental activists, notable Nobel Peace Prize nominee Ken Saro-Wiwa, made international headlines and brought world-wide recognition of the serious environmental degradation of Nigeria [ 9 ]. Today, the oil-rich Niger Delta region of Nigeria is always the first point of reference when analysing the Nigerian environment. This stems from the fact that despite the sacrifice of Ken Saro-Wiwa and others, much has not changed in terms of making the environment pollution free. In fact, it has even gotten worse with time and recent developments. During the 1990s, the Niger Delta locals learned that extortion pays. Villagers found that by sabotaging oil installations to collect oil spill compensation from shell (an oil firm) they could earn more than by marginal subsistence farming on degraded lands. Thus, sabotaging and spills became a new dimension of increasing crude oil pollutants. Attacks on oil facilities and pipelines became even more relentless, and the Niger River delta was an increasingly bloody place. Environmental degradation from crude oil productions continued, and by 1999 the United Nations declared the delta the most threatened in the world [ 9 ]. In early 2006, conditions worsened in the delta. The number of kidnapping of oil workers increased as did attacks on oil facilities. The Niger Delta is made up of six states of the south-south region, namely, Bayelsa, Akwa Ibom, Cross River, Delta, Rivers, and Edo.

This lingering problem caused the former President of Nigeria, Alhaji Umaru Musa Yar'adua, to create a Ministry of the Niger Delta so as to oversee the well-being of the environment and the people of this region. Currently more is being done in the Niger Delta to bring about reduction in vandalization of oil pipelines as well as an amnesty for the irate youths in this part of Nigeria in order to find a lasting solution to both the social and environmental problem of the Niger Delta area of Nigeria.

Crude oil spill affects germination and growth of some plants [ 10 ], it also affects the overall production of crop (e.g., Zea mays ) due to its negative impact on the chlorophyll content which is a marker of the yield of plants [ 11 ]. Severe crude oil spill in Cross River state, Nigeria, has forced some farmers to migrate out of their traditional home, especially those that depend solely on agriculture. The negative impact of oil spillages remains the major cause of depletion of the Niger Delta of Nigeria vegetation cover and the mangrove ecosystem [ 12 ].

4.2. The Nigerian Environment: Case Study of Solid Waste Generation in Cities

The Nigerian cities such as Aba, Enugu, Onitsha, Kano, Ibadan, and Lagos are characterised by huge mounds of solid waste dumps generated from households, industries, markets, schools, and street trading. This can be attributed to migration, population increase, urbanization, constructions, and industrialization coupled with inefficient, improper and some times nondisposal of wastes. Solid waste dumps are indiscriminately formed on streets, homes, road side, markets, and other places where human activities take place in the cities.

Solid wastes can be broadly grouped into two as it relates to the concept of this write up. These two categories are the following.

(a) The Biodegradables (Biowastes) —

These include those solid wastes generated, which could be decomposed by microorganisms and does not constitute major sources of pollution for a long period of time. They are paper products (such as printing papers, waste books, newspapers, carton, toilet paper, card boards), and wastes of plant origin (fruits, stems, roots, vegetables, leaves, food remains and garden solid wastes, etc.), wastes of animal origin (faecal matter, carcass, droppings, and poultry waste products). These groups of solid waste even though they are easily degraded by microorganism in minimal time, give off offensive odour and constitute nuisance to the aesthetic environment more than the nonbiodegradable solid wastes. They can also constitute a good habitat for the thriving of pathogenic microorganisms which could easily pollute fresh food product and sources of fresh water in the urban cities in Nigeria.

(b) Nonbiodegradable (Rubbish/Garbage) —

These groups of solid wastes are not degradable or hardly degraded by microorganisms. Hence, other means of treatment such as incineration, land refill, and recycling are currently employed in Nigeria as ways of disposing them. Examples of this group of solid wastes are solid wastes of metallurgical and smelting industries (abandoned vehicles, motor cycles, vehicle part and scrap metals, iron, zinc, aluminium sheets and other metals, machine parts); solids wastes of construction industries (sand, gravel, bitumen wastes, concrete and waste building materials); solid waste of plastic industries (plastic buckets, cable insulators, tyres, chairs, tables, cellophane bags, plastic bottles, cutleries, sachet water containments, etc.) and glass products. These might not give out offensive odour, but they are even worse nuisance to the environment since their disposal has become a “Herculean” and near-impossible task in Nigeria.

Solid waste management activities include prevention (pollution prevention from sources), source reduction (pollution minimization in waste generating activities at point of good production), and treatment (safe disposal of nonrecyclable residues, recycling, transport of waste to land refills).

The major problem is that Nigeria is yet to develop efficient ways of waste disposal which are eco-friendly and which could be recycled back into the environment without constituting nuisance to the environment or affecting the health of the biotic components of the ecosystem.

4.3. Environmental Degradation due to Mining Activities in Nigeria

The natural topography of many cities and country side in Nigeria had been destroyed as a result of commercial activities involved in the exploration and exploitation of numerous minerals that abound in the country. Places like Jos, Bauchi, Nasarawa, and Enugu states have been worst affected by environmental degradation which had defaced the beautiful landscape of the natural environment.

No consistent mining regulatory law is enforced in the country. The exploration of tin in the Plateau (Jos) started as early as 1808 by the British colonialists, and in the 1970s Nigeria produced an average of 10,000 tons of tin ore annually. Output fell to 3,000 tons in the 1980s and dropped again to 500 tons in the 1990s. Nigeria now earns less than 0.5% of its foreign exchange from tin [ 13 ]. For over 70 years Jos tin mining industry was mostly controlled by overseas companies. But when the company was nationalised in 1972, no one took responsibility for clearing up the mess left behind. In places on the plateau such as Bukuru, Rayfield, Barkin Ladi, Mangu, Anglo Jos, Zawan, Du, Shen, Gyel and Shere Hills, ugly gashes left over from past mining activities can be seen everywhere. Alarmingly, effluents from nearby industries have seeped deep into mines-turned-water holes. Farmers use water from dams which resulted from tin mining activities for irrigation. The top soil also washes into streams in neighbouring village water that is used for drinking and other domestic purposes.

In addition, locals use soil left over from the abandoned mining sites—containing naturally found radioactive heavy metals to build houses. Environmentalists fear that people living in these houses risk being exposed to unhealthy levels of radiation [ 13 ].

Tin mining has also displaced many people from fertile agricultural land. The mining sites are located in the best areas in terms of the terrain and the flatness of the land. The people are now compelled to farm on rocky land. Government on its part instead of reclaiming the lands and resettling the people only asked people whose lands are destroyed to move out of these danger zones without compensation or arrangements to resettle them properly.

Thus, the tin areas have environment whose topography is made up of dams (which claim lives of both human and animals annually) as well as ‘‘a lunar landscape of steep-sided mounds with multicoloured ponds or lakes” [ 13 ].

Nigerian environmentalists have agreed that mining activities such as tin-Jos, Coal-Enugu, and others have done great damages to the environment which will need a concerted effort especially adoption of better mining practices in order to remediate.

4.4. Erosion, Desertification, and Deforestation: Loss of Biodiversity in the Nigerian Environment

Erosion problem is also a major environmental threat in Nigeria as sheet and gully erosion have wrecked untold havoc in several states such as Abia, Adamawa, Anambra, Delta, Ebonyi, Edo, Enugu, Gombe, Jigawa, Kogi, Ondo, Ogun, and Lagos. In Lagos state and other coastal areas, coastal erosion has destroyed properties and valuable lands were washed away. Most of the flooding and erosion seen in cities are as a result of poor drainage system.

Places such as the eastern states of Nigeria (Anambra, Imo, Abia, Enugu, and Ebonyi) have regions prone to erosion. This has resulted in the entire loss of farm land and buildings. The situation is so pathetic that a whole clan in a southern part of Anambra state was forced to take refuge in a primary school. Places like Agulu and most part of Aguata and Orumba Local government areas are highly endangered with erosion invasion. The Northern part of the country has ecosystem characterised by the Savannah clime. Starting from the North Central region encompassing Benue to Katsina states in the farthest part of the North made up of the southern savannah, Northern savannah, Sudan, and Sahel savannah characterized by low foliage and little trees. The environment is marked by constant grazing and building of huts which affect the type of plant survival as well as desert encroachment from the Niger and the Chad republics at the furthest part of the country.

Deforestation is a serious problem in Nigeria, which currently has one of the highest rates of forest loss (3.3 Percent) in the world. Since 1990, the country has lost some 1 million hectares or 35.7 percent of its forest covers [ 9 ]. Worse Nigeria's most biodiverse ecosystems—its old-growth forests is that are disappearing at an even faster rate. Between 1990 and 2005, the country lost a staggering 79% of these forests and since 2000 Nigeria has been losing an average of 11 percent of its primary forests per year—double the rate of the 1990s. These figures mark Nigeria as having the highest deforestation rate of natural forest on the planet. As its forests fall, Nigeria has seen wildlife populations plummet downward from poaching and habitat loss, increasing desertification. It appears that Nigeria's swift economic development has exacted a high toll on its people and environment [ 9 ].

The problems of environmental degradation have continued to plague Nigeria, and they have defied proffered solution mainly due to improper applications and also the lack of proper waste control and environmental maintenance. The major causes of environmental degradation problems were identified by the Vision 2010 Committee set up by the Federal Government. Aina and Salau [ 14 ] enumerated some of these problems as follows:

• poverty as a cause consequence of environmental exploitation, with the poor scavenging marginal lands to eke out a living;
• bush burning for farming and ever-increasing depletion of young forests for fuel wood.
• uncontrolled logging accentuated by lack of re-stocking in many parts of the country. This practice is linked with the loss of precious biological diversity (nature's gene bank of raw materials for future development);
• gas flaring, Crude oil spill and the resultant problem of ecosystem destabilization, heat stress, acid rain and acid precipitation-induced destruction of fresh water fishes and forests in the coastal areas of the country. Nigeria alone accounted for about 28% of the world's total gas flared;
• a general inability of the agencies responsible for the environment to enforce laws and regulations, particularly with respect to urban planning and development, prospecting for minerals and adherence to industrial standards, sitting of public and residential quarters in flood-prone areas, unsettled dump site improperly reclaimed and converted to plots.

5. The Need for Pollution Prevention

Most of the pollutants in the environment are directly or indirectly the product of industrial activities/production. Awareness of the deleterious effect of pollutants in the environment is on the increase. Government, environmentalists, and communities for a long time have been frowning at the degradation of the environment due to man-made pollutants especially those that are by-product of industries. Industries on the other hand are under pressure by their communities to minimize the pollutants they generate. This has placed the manufacturing industries at high cost of revenue for pollution treatment as well as Billion of Naira for research into eco-friendly ways of manufacturing processes which minimizes pollution generation. Most of the pollution released from industrial processes includes discharge into the environment, namely: air, land and water. The best points of pollution prevention involves, source reduction (by using raw materials more efficiently); pollution control (substituting less harmful substances for hazardous materials); pollution management (eliminating toxic substances from the production process).

By implementing pollution prevention practices, companies often reduce their operational waste disposal, and compliance costs ( http://www.p2.org/about/nppr_p2.cmf/ ).

6. Biotechnology: The Hope for Environmental Sustainability

As earlier stated, man's activities in his environment involve a lot of chemical synthesis in the process of converting the natural products in his environment into other forms convenient for his consumption. In the quest for converting wood into timber, use of fruits in juice production, use of herb for drug synthesis, conversion of petrochemical substances into polythene products, the environment correspondingly becomes littered with substances not needed in the cause of production. In the process of creating products, man also creates problems either consciously or unconsciously vis-à-vis pollution. As a result, the most acceptable solution to the generated wastes in the environment is such that will conveniently integrate them back into the environment. That method involves the use of microorganisms—usually yeasts, bacteria, or fungi as whole cell usage production system or in the form of industrial enzymes. In many cases these microorganisms or their products are integrated into the substrates which give us the products, desired in the industries, examples of these are bioleaching (biomining), biodetergent, biotreatment of pulp, biotreatment of wastes (bioremediation), biofiltrations, aquaculture treatments, biotreatment of textiles, biocatalysts, biomass fuel production, biomonitoring, and so forth. These are biotools (biotechnological tools), which could solve the problem of pollution and help sustain the environment. This is so because when the products or their constituents are discarded, they go back into the ecosystem. As such, they become reconverted into organic components of the environments. Moreover, their production is strictly biological instead of chemical (synonymous to pollution introduction).

These biotechnology tools have long been used in many developed countries in the world such as the United States, Finland, Sweden, Germany, Japan, and others. Africa is still lagging from being integrated into these environmental sustainability best practices. Nigeria is the focus on how to begin to make use of these biotools for the improvement of the badly degraded environment.

7. Biotechnology

Biotechnology is defined as a set of scientific techniques that utilize living organisms or parts of organisms to make, modify, or improve products which could be plants or animals. It is also the development of specific organisms for specific application or purposes and may include the use of novel technologies such as recombinant DNA, cell fusion, and other new bioprocesses [ 15 ].

Biotechnology is not new; it has been employed for centuries in the production of fermented foods such as gari, bread, yoghurt, and cheese and beverages such as wine and beer [ 16 ]. Thus, it is a natural phenomenon in use even in Africa (Nigeria) though its principle was not well understood. CTA [ 16 ] report illustrated the denomination of “green,” “red” and “white” biotechnology according to its uses and applications. Moreover, Disilva [ 17 ] has a different classification as shown in Table 1 .

Classification of biotechnologies. Modification from: Disilva [ 17 ].

Despite the that classification for convenience, using the CTA [ 16 ] classification encompasses all others. As a result “Green biotechnology” encompasses a wide range of techniques that consists of culturing plant tissues and/or organs, followed by the multiplication of the relevant plants with desirable characteristics. Genetically identical plantlets are thus available for distribution to farmers, horticulturalists, forestry growers, and nurseries all the year round. It also includes the transformation of plants, crop species, and varieties through genetic engineering techniques, leading to what are known as “genetically modified” (GM) crops. In addition, green or agricultural biotechnology also applies to techniques used in livestock husbandry (nutrition and reproduction). Green biotechnology should therefore not only be equated with advances in genetic engineering.

“Red biotechnology” encompasses the genetic engineering technique that has been used since the mid-1970s to produce drugs and vaccines in microorganisms, animal cells, and more recently in plants. For example, insulin, human and bovine growth hormones, interferon, cell growth factors, antihepatitis B vaccine, and others are being produced in this way. A wide range of diagnostic techniques and veterinary vaccines are produced using red or medical biotechnology [ 16 ].

“White biotechnology” refers to a wide range of processes resulting in fermented products and chemicals (e.g., enzymes, biofuels such as ethanol and bioplastics) as well as to the technologies used in recycling waste water, industrial effluents, and solid wastes. These “bioremediation” processes contribute to the abatement of pollution. The extraction of metals from ores with the help of microorganisms (biomining) is also part of white or environmental biotechnology [ 16 ].

8. Environmental Biotechnology

Environmental biotechnology is “the integration of natural sciences and engineering in order to achieve the application of organisms, cells, parts thereof and molecular analogues for the protection and restoration of the quality of our environment” [ 18 ].

The nomenclature of “white biotechnology” is alluded to both industrial and environmental biotechnology. Biotechnological tools for environmental sustainability are qualified to be greatly associated as major component of the “White biotechnology.”

Biotechnological processes to protect the environment have been used for almost a century now, even longer than the term “biotechnology” exists [ 18 ]. Municipal sewage treatment plants and filters to purify town gas were developed around the turn of the century. They proved very effective although, at the time, little was known about the biological principles underlying their function. Since that time, our knowledge base has increased enormously [ 18 ].

Biotechnological techniques to treat waste before or after it has been brought into the environment are components of environmental biotechnological tools. Biotechnology can also be applied industrially for use in developing products and processes that generate less waste and use less nonrenewable resources and consume less energy. In this respect biotechnology is well positioned to contribute to the development of a more sustainable society through a sustainable environment. Recombinant DNA technology has improved the possibilities for the prevention of pollution and holds a promise for a further development of bioremediation [ 18 ]. What this means for environmental biotechnology is that it is futuristic and limitless in application and usage.

9. Biotechnological Tools for Environmental Sustainability

Biotools for the sustenance of the environment are those biotechnological processes that make use of bioproducts as well as microorganisms for pollution reduction, production of environmental friendly products as well as general maintenance of the pristine (natural) environment for the benefit of man and other ecosystem components. It is an aspect of environmental biotechnology concerned with prevention of processes capable of causing an unsustainable environment for man and ecocomponents. Some of the biotools in use will be briefly and concisely enumerated here, and it is by no means exhaustive due to current and future addition to the body of knowledge in the environmental biotechnology field. The discussion will centre on the current or future projection of the usage of these tools elsewhere and the need for Nigeria and other countries which are hitherto not adapting to their usage due to environmental and technological limitations to break such barriers and begin in earnest to adopt their usage. Some of the biotools are as enumerated in the following.

9.1. Biodetergents and Biosolvent Research/Production

FAIR [ 19 ] stated that solvents and detergents are important in a number of industries, and most are derived from petroleum. There is increasing concern that prolonged contact with solvents causes health problems for workers in the factories where solvents and detergents are produced and for those using such substances in domestic and commercial laundry in their everyday work. The aim for research into biodetergents is to create biological substitutes for solvents and detergents derived from petroleum. Apart from detergents, research is also ongoing into developing substitutes of biological origin for solvents used in the production of paints, offset-printing ink, and so forth. These new solvents will be made by mixing together several common bioliquids: such as bioethanol, terpenes, vegetable oils, fatty acids, methyl esters, and derivatives of related compounds [ 19 ].

The key to success is mixing the right compounds together in the right proportions. To do this, researchers are developing mathematical models, which will allow the “recipes” for the new biosolvents to be optimised. The mixtures being developed are designed to meet the criteria established by the companies participating in the project. Those small- and medium-sized enterprises from France, the United Kingdom, The Netherlands, Denmark, and Belgium are paint producers, manufacturers of ink for use in offset printing, and producers of detergent. Their activities are examples of only three industries where solvents are used: the application of biosolvents to other industrial processes will also be promoted as part of this project [ 19 ].

9.1.1. Benefits for Farmers, Workers, and the Environment

FAIR [ 19 ] SMEs research release reported that most solvents and detergents in current use are derived from petroleum, as a result there are two major drawbacks to these solvents and detergents compared to the biological counterparts. First, one day the crude oil from which they are derived will no longer be available since they are nonrenewable natural resources and again they present a major waste disposal problem as they do not break down readily when disposed of hence pollute the environment.

Presently [ 19 ], the success of the new biosolvents and detergents are limited since they are more expensive than the traditional products. At the moment, conventional solvents are cheap and the replacement bioproducts could not compete on price. But as governments start to use taxation to stimulate the use of renewable resources, economic viability of biosolvents should improve. Increased use of biosolvents and biodetergents will stimulate demand for the raw materials used to manufacture the new products and so help farmers by creating a new outlet for their produce. The new solvents and detergents being developed will have a much less detrimental effect than existing products on the health of workers making and using them. Agricultural product, job creation, and environmental sustainability are the benefits accruable due to this biotool innovation. Development of the product will help lessen dependence on non-renewable resources (prevents nonrenewable environmental exploitation), safeguard human health, and protect the environment from chemical pollutants seeping into aquatic body and atmosphere.

Nigeria can take a cue from this giant stride and begin its own processing of the natural and agricultural product as well as make use of the fertile land to develop bio products friendly to the environment. It is a viable project friendly to the environment. It is a viable project which can be adopted since it requires less energy consumption and minimal technological effort. Hence, Nigerian government, environmentalists, industrialists, and research institution can adopt this novel production of biodetergent and biosolvents for its environmental sustainability.

9.2. Bioremediation

Bioremediation is the use of biological systems for the reduction of pollution from air or from aquatic or terrestrial systems [ 18 ], it also involves extracting a microbe from the environment and exposing it to a target contaminant so as to lessen the toxic component [ 20 ]. Thus, the goal of bioremediation is the employment of biosystems such as microbes, higher organisms like plants (phytoremediation) and animals to reduce the potential toxicity of chemical contaminants in the environment by degrading, transforming, and immobilizing these undesirable compounds.

Biodegradation is the use of living organisms to enzymatically and otherwise attack numerous organic chemicals and break them down to lesser toxic chemical species. Biotechnologists and bioengineers classify pollutants with respect to the ease of degradation and types of processes that are responsible for this degradation, sometimes referred to as treatability [ 20 ].

Biodegradation with microorganisms is the most frequently occurring bioremediation option. Microorganisms can break down most compounds for their growth and/or energy needs. These biodegradation processes may or may not need air. In some cases, metabolic pathways which organisms normally use for growth and energy supply may also be used to break down pollutant molecules. In these cases, known as cometabolisms, the microorganism does not benefit directly. Researchers have taken advantage of this phenomenon and used it for bioremediation purposes [ 18 ].

A complete biodegradation results in detoxification by mineralising pollutants to carbon dioxide (CO 2 ), water (H 2 O), and harmless inorganic salts [ 18 ]. Incomplete biodegradation (i.e., mineralization) will produce compounds that are usually simpler (e.g., cleared rings, removal of halogens), but with physical and chemical characteristics different from the parent compound. In addition, side reactions can produce compounds with varying levels of toxicity and mobility in the environment [ 20 ].

Biodegradation may occur spontaneously, in which case the expressions “intrinsic bioremediation” or “natural attenuation” are often used [ 18 ]. In many cases the natural circumstances may not be favourable enough for natural attenuation to take place due to inadequate nutrients, oxygen, or suitable bacteria. Such situations may be improved by supplying one or more of the missing/inadequate environmental factors. Extra nutrients [ 18 ] were disseminated to speed up the break down of the oil spilled on 1000 miles of Alaskan shoreline by the super tanker Exxon Valdez in 1989.

According to Vallero [ 20 ], there are millions of indigenous species of microbes living at any given time within many soil environments. The bioengineer simply needs to create an environment where those microbes are able to use a particular compound as their energy source. Biodegradation processes had been observed empirically for centuries, but putting them to use as a distinct field of bioremediation began with the work of Raymond et al. [ 21 ]. This seminal study found that the addition of nutrients to soil increases the abundance of bacteria that was associated with a proportional degradation of hydrocarbons, in this case petroleum by-products [ 21 ].

9.2.1. Life Chemical Dynamics (Biochemodynamics) of Bioremediation

Bioremediation success [ 20 ] depends on the following:

• the growth and survival of microbial populations; and
• the ability of these organisms to come into contact with the substances that need to be degraded into less toxic compounds;
• sufficient numbers of microorganisms to make bioremediation successful;
• the microbial environment must be habitable for the microbes to thrive.

Sometimes, concentrations of compounds can be so high that the environment is toxic to microbial populations. Therefore, the bioengineer must either use a method other than bioremediation or modify the environment (e.g., dilution, change of pH, pumped Oxygen, adding organic matter, etc.) to make it habitable. An important modification is the removal of non-aqueous-phase liquids (NAPLs) since the microbes' biofilm and other mechanisms usually work best when the microbe is attached to a particle; thus, most of the NAPLs need to be removed, by vapour extraction [ 20 ]. Thus, low permeability soils, like clays, are difficult to treat, since liquids (water, solutes, and nutrients) are difficult to pump through these systems. Usually bioremediation works best in soils that are relatively sandy, allowing mobility and greater likelihood of contact between the microbes and the contaminant [ 20 ]. Therefore, an understanding of the environmental conditions sets the stage for problem formulation (i.e., identification of the factors at work and the resulting threats to health and environmental quality) and risk management (i.e., what the various options available to address these factors are and how difficult it will be to overcome obstacles or to enhance those factors; that make remediation successful). In other words, bioremediation is a process of optimization by selecting options among a number of biological, chemical and physical factors these include correctly matching the degrading microbes to conditions, understanding and controlling the movement of the contaminant (microbial food) so as to come into contact with microbes, and characterizing the abiotic conditions controlling both of these factors [ 20 ]. Optimization can vary among options, such as artificially adding microbial populations known to break down the compounds of concern. Only a few species can break down certain organic compounds [ 20 ]. Two major limiting factors of any biodegradation process are toxicity to the microbial population and inherent biodegradability of the compound. Numerous bioremediation projects include in situ (field treatment) and ex situ (sample/laboratory treatment) waste treatment using biosystems [ 20 ].

Table 2 shows the application of bioremediation in various environmental processes.

Environmental process and bioremediation procedures involved.

10. A Practical Application of Microorganism in Crude Oil Bioremediation

According to Onwurah [ 22 ] many microorganisms can adapt their catabolic machinery to utilize certain environmental pollutants as growth substrates, thereby bioremediating the environment. Some microorganisms in carrying out their normal metabolic function may fortuitously degrade certain pollutants as well. This process termed cometabolism obviously requires adequate growth substrates. Diazotrophs, such as Azotobacter vinelandii , beyond their ability to fix atmospheric nitrogen also have the capacity, in some case, to cometabolise petroleum hydrocarbons [ 10 ].

Onwurah [ 22 ] carried out a bioremediation study that involved two bacteria, a hydrocarbonoclastic and diazotrophic bacteria. The hydrocarbonoclastic was tentatively identified as Pseudomonas sp. and designated as NS 50 C 10 by the Department of Microbiology, University of Nigeria, Nsukka. The diazotrophic bacteria was Azotobacter vinelandii , which was isolated from previously crude oil-contaminated soil [ 10 ]. This study describes the mineral media and procedure for isolation and multiplication of the bacteria to the required cell density. Crude oil spill was simulated by thoroughly mixing 50, 100, and 150 mg fractions of crude oil with 100 g batches of a composite soil sample in beakers. The soil samples were taken from a depth of 0–50 cm from the Zoological garden, University of Nigeria, Nsukka. The mixing was conducted using a horizontal arm shaker adjusted to a speed of 120 rpm for 30 minutes. The contaminated soil samples, in beakers, were inoculated with optimal combinations (cell density) of NS 50 C 10 and A. vinelandii . Water was added to the crude oil-contaminated soil samples (both inoculated and those not inoculated to a saturation point but not in excess), and then the samples were left to stand undisturbed for seven days. NS 50 C 10 was applied first, followed by A. vinelandii , 12 hours later. At the seventh day of soil treatment, 20 sorghum grains (previously soaked overnight in distilled water) were planted in each soil sample followed by irrigation to aid germination. Seven days after the planting of the sorghum grains, the soil from each beaker was carefully removed. The number of germinated seed per batch of soil sample was noted, the length of radicules was measured, and the mean length was taken from each batch.

The results of this experiment showed that Pseudomonas sp. grew well on agar plates containing a thin film of crude oil as the only carbon source, while A. vinelandii did not. However, cell-free extract of Azotobacter vinelandii fixed atmospheric nitrogen as ammonium ion (NH 4 + ) under appropriate condition. The specific growth rate values in contaminated soil samples inoculated with both normal NS 50 C 10 and A. vinelandii (consortium) were highest in all cases. By adding an aerobic, free living diazotroph A. vinelandii with the Pseudomonas sp. (NS 50 C 10 ), an improvement on bioremediation of soil over that of the pure NS 50 C 10 alone was achieved to the order of 51.96 to 82.55%. This innovative application that uses the synergetic action of several microorganisms to clean up oil-polluted soil has potential application for the bioremediation of oil-contaminated soil in the Niger delta region.

The method described above is the biotechnological application known as bioaugmentation which is the addition of selected organisms to contaminated soils (sites) in order to supplement the indigenous microbial population and speed up degradation. Figure 1 presents a model of the process involved in this bioremediation technique.

Simplified bioremediation conceptual model of Pseudomonas sp. and A. vinelandii operating as a unit of two miniature sequencing bioreactors, in situ (SMP: soluble microbial products; N-cpd: fixed nitrogen compounds; EPS: exopolysaccharide; PHC = petroleum hydrocarbons) [ 22 ].

This bioremediation method by the authors has been applied in bioremediation especially in Niger delta areas of Nigeria. The authors also serve in the capacity of industrial consultants in the specialized field of crude oil pollution clean-up procedures using this specific biotool (bioremediation).

10.1. Biofiltration

This is a pollution control technique employing the use of living material to capture and biologically degraded process pollutants. Common uses of biofiltration processes are for processing waste water, capturing harmful chemicals or silt from surface runoff, and microbiotic oxidation of contaminants in air ( http://www.biofilter.com/ ).

In multimedia-multiphase bioremediation, waste streams containing volatile organic compounds (VOCs) may be treated with combinations of phases, that is, solid media, gas, and liquid flow in complete biological systems. These systems are classified as three basic types: biofilters, biotrickling filters, and bioscrubbers ( http://www.biofilter.com/ ). Biofilms of microorganisms (bacteria and fungi) are grown on porous media in biofilters and biotrickling systems. The application of this biotechnological tool includes the following.

10.1.1. Control of Air Pollution

When applied to air filtration and purification, biofilters use microorganisms to remove air pollution ( http://www.biofilter.com/ ). The air flows through a packed bed, and the pollutant transfers into a thin biofilm on the surface of the packing material. Microorganisms, including bacteria and fungi, are immobilized in the biofilm and degrade the pollutant. Trickling filters and bioscrubbers rely on a biofilm and the bacterial action in their recirculating waters ( http://www.biofilter.com/ ). The air or other gas containing the VOCs is passed through the biologically active media, where the microbes break down the compounds to simpler compounds, eventually to carbon dioxide (if aerobic), methane (if anaerobic), and water. The major difference between biofiltration and trickling systems is how the liquid interfaces with the microbes. The liquid phase is stationary in a biofilter ( Figure 2 ), but liquids move through the porous media of a biotrickling system (i.e., the liquid “trickles”).

Schematic of packed bed biological control system to treat volatile compounds. Air containing gas phase pollutants (CG) traverse porous media. The soluble fraction of the volatilized compounds in the air steam partition into the biofilm (CL) according to Henry's Law. CL = {CG/H} where H is Henry's Law constant. Adapted and modified from Vallero [ 23 ].

A particular novel biotechnological method in biofilteration ( Figure 3 ) uses compost as the porous media. Compost contains numerous species of beneficial microbes that are already acclimated to organic wastes. Industrial compost biofilters have achieved removal rates at the 99% level [ 20 ]. Biofilters are also the most common method for removing VOCs and odorous compounds from air streams.

Biofiltration without a liquid phase used to treat vapour phase pollutants. Air carrying the volatilized contaminants upward through porous media (e.g., compost) containing microbes acclimated to break down the system can be heated to increase the partitioning to the gas phase. Microbes in the biofilm surrounding each individual compost particle metabolize the contaminants into simpler compounds, eventually converting them into carbon dioxide and water vapour. Modified from Vallero, [ 20 ].

In addition to a wide assortment of volatile chain aromatic organic compounds, biological systems have successfully removed vapour-phase inorganics, such as ammonia, hydrogen sulfide, and other sulfides including carbon disulfide, as well as mercaptans. The operational key is the biofilm. The gas must interface with the film. Compost has been a particularly useful medium in providing this partitioning [ 20 ]. Industries employing the biofiltration technology include food and animal products, off-gas from waste water treatment facilities, pharmaceuticals, wood products manufacturing, paints, and coatings application and manufacturing and resin manufacturing and application. Compounds treated are typically mixed VOCs and various sulfur compounds, including hydrogen sulfide ( http://www.biofilter.com/ ). Maintaining proper moisture condition is an important factor in biofiltration. The air normally humidifies before it enters the bed with a watering (spray) system, humidified chamber, bioscrubber, or biotrickling filter. Properly maintained, a natural organic packing media peat, vegetable mulch, bark, or wood chips may last for several years. However, engineered combined natural organic and synthetic component packing materials will generally last much longer, up to 10 years. A number of companies offer these types or proprietary packing materials and multiyear guarantees, not usually provided with a conventional compost or wood chip bed biofilter. For large volumes of air, a biofilter may be the only cost effective solution ( http://www.biofilter.com/ ). There is no secondary pollution (unlike the case of incineration where additional CO 2 , CO, and NO gases are produced from burning fuel(s) and degradation products form additional biomass, carbon dioxide and water).

10.1.2. Water Treatment

Trickling filters have been used to filter water for various end uses for almost two centuries. Biological treatment has been used in Europe to filter surface water for drinking purposes since the early 1900s and is now receiving more interest worldwide ( http://www.biofilter.com/ ). Media irrigation water, although many systems recycle part of it to reduce operating costs, has a moderately high biochemical oxygen demand (BOD) and may require treatment before disposal. Biofilters are being utilized in Columbia falls, Montana at Plum Creed Timber Company's fibreboard plant ( http://www.biofilter.com/ ).

Biofiltration is one of the most effective water treatment technologies. Its application includes water filtration in farms, livestock operations, city municipal, industrial, and household applications. Some of the organisations which have supported the development or application biofiltration of water ( http://www.biofilter.com/ ) over the past 14 years include the following:

• Prairie Farm Rehabilitation Administration (PFRA),
• The National Research Council (NRC),
• The Saskatchewan Research Council (SRC),
• Napier University (Scotland),
• Agriculture and Agro-food Canada.

Biofiltration is ideal for well, lake, pond, river, and dug out water. Biofilters remove the following substances from air and water: iron and iron bacteria, parasites, colour, cysts, manganese, pesticides, arsenic, lead, mercury, turbidity, dissolved organic carbon (dissolved organic material in water), tannins [ 26 ].

A good number of research and practical work has been and is being carried out by Nigerian scientists and academics in the area of biofiltration of waste water. Bearing in mind that good water is a very essential commodity which is not readily available in most part of the country, it is therefore of great necessity to look at economic feasible ways to treat water for the benefit of the citizens. The sources of portable water for most Nigerian cities are government treated tap water and commercially treated drinking water as well as domestic water by water service private firms. Rural communities make do with water from ponds, streams, rivers, rain, and spring which are prone to contamination by water-borne diseases such as typhoid and diarrhoea which is common in those communities. Some of the applications of this important biotool in Nigeria are as follows.

• Asamudo et al. [ 27 ] demonstrated the effectiveness of using the fungus Phanerochaete chrysosporium in the biofiltration of textile effluent, polycyclic aromatic hydrocarbons (PAH), and pulp and paper effluents. The microorganism was capable of producing extracellular enzymes such as manganese peroxidase, cellulases, and lignin peroxidases, in achieving total remediation of these effluents.
• Ezeronye and Okerentugba [ 28 ] carried out a study to demonstrate the effectiveness of a yeast biofilter composed of a mixed culture of Saccharomyces spp., Candida spp., Schizosaccharomyces spp. and Geotrichum candidum in the treatment of fertilizer factory effluents and 98% treatment efficiency was achieved. The biochemical oxygen demand (BOD) of the effluent was reduced from a range of 1200–1400 to 135–404 mg/L. Besides, ammonia nitrogen (NH 3 -N) and nitrate-nitrogen (NO 3 -N) were reduced from 1000–10 mg/L and 100–17.6 mg/L, respectively.
• Ogunlela and Ogunlana [ 29 ] developed a system using lava stones and oyster shells biofilter substrates for the oxidation of ammonia in a recirculatory aquaculture system. The effluent was treated using the biofilter, and chemical analyses were carried out once a week for four consecutive weeks. The results at the end of the fourth week indicated that the ammonia and nitrite concentrations were 0.0374 mg/L and 0.292 mg/L, respectively, which were below the permissible limits of 0.05 mg/L and 0.3 mg/L for ammonia and nitrite, respectively.

One of the most recent innovations in the use of this biotool in Nigeria was by Rabah et al. [ 30 ]. Their work describes the use of yeast biofilters in the treatment of abattoir waste water. Thus, Nono (locally fermented milk product) and Kunun-zaki (a refreshing drink made from millet) samples were obtained at the minimarket of the main campus of the Usmanu Danfodiyo University, Sokoto, Nigeria, in sterile sample bottles and transported in an icebox to the laboratory for the isolation of yeasts. Wastewater was collected from an abattoir in Sokoto, Nigeria, using sterile two litre capacity sample bottles and transported in an icebox to the laboratory. The wastewater was collected from three points in the abattoir: at the point where the wastewater leaves the slaughter hall (Point A, PA), midway through the drainage channel (Point B, PB), and the point where the wastewater drained to the surrounding soil (Point C, PC). A total of three samples were collected from each point at different times.

The biofilter was constructed using Perspex glass with a length of 18.0 cm, width of 10.8 cm, and a depth of 10.5 cm. The filter has upper and lower compartments separated by a perforated partition made up of the same Perspex glass. It also has a tap for the collection of filtered wastewater. Potato peels were ground to smaller particles, wetted, and placed on the perforated partition. The yeast biomass was inoculated on the peels and left for one week at ambient laboratory temperature (28 ± 2°C) to allow the cells to grow. Then the abattoir wastewater was introduced into the filter bed and left to stand for a minimum period of 14 days. The filtered wastewater was collected from the lower chamber of the filter through a tap fitted to the chamber.

The yeast species isolated from the Nono and Kunun-zaki and identified for use as biofilters in the biofiltration process were identified as Candida krusei, Candida morbosa, Torulopsis dattila, Torulopsis glabrata, and Saccharomyces chevalieri. Also the results of the physicochemical qualities of the abattoir wastewater before and after biofiltration process from the three sampling points (PA, PB, and PC) revealed that there was a considerable reduction in pH, nitrate (NO 3 ), dissolved oxygen (DO), biochemical oxygen demand (BOD), and chemical oxygen demand (COD) after the biofiltration of the wastewater collected from the three sampling points. It was also observed that the concentrations of other compounds in the wastewater varied with the sampling points probably due to contamination from human activities in the abattoir such as dumping of cow dung and pieces of bones in the wastewater channels. According to Rabah et al. [ 30 ], the results generally indicated that the yeast biofilter was fairly effective in the bioremediation process. The biofilter had a percentage efficiency of 42.5%.

10.2. Biomining

Bacteria leaching is now used throughout the world as an additional technique for extracting metals from ores. Metals which can be extracted in this way include copper, uranium, cobalt, lead, nickel, and gold [ 31 ].

Biomining is a generic term [ 32 ] that describes the processing of metal-containing ores and concentrates of metal containing ores using microbiological technology. Biomining has application as an alternative to more traditional physical-chemical methods of mineral processing. Commercial practices of biomining can be broadly categorized in two, namely, mineral biooxidation and bioleaching. Both processes use naturally occurring microorganisms to extract metals from sulphide bearing minerals. Minerals biooxidation refers to the process when it is applied to enhance the extraction of gold and silver, whereas bioleaching usually refers to the extraction of base metals, such as Zinc, Copper, and Nickel.

Collectively, minerals biooxidation and bioleaching are commercially proven, biohydrometallurgical or biomining processes that are economic alternatives to smelting, roasting, and pressure oxidation to treat base and precious metals associated with sulphide minerals [ 32 ].

Metals are essential physical components of the ecosystem, whose biologically available concentrations depend primarily on geological and biological processes [ 33 ]. Elevated levels of metals at specific sites can create a significant environmental and health problem when the release of metals through geological processes of decomposition and anthropogenic processes far exceeds that of natural processes of metal cycling. Metal contamination of both aqueous and terrestrial environments is of great concern, due to the toxicity and persistence of metals in the ecosystem and their threat to animal and human health [ 34 ]. Bacteria play an important role in the geochemical cycle of metals in the environment, and their capabilities and mechanisms in transforming toxic metals are of significant interest in the environmental remediation of contaminated sites. Microorganisms [ 34 ] colonize and shape the Earth in many ways, and their ability to adsorb and transform metals can shade light on solving pollution problems and proposing solutions in the clean up of contaminated site.

10.2.1. Extraction Role of Microbes in Biomining

Although many undiscovered microbial communities are involved in biomining, some of the popular and discovered bacteria responsible are: Leptospirillum ferrooxidans , Acidithiobacillus thiooxidans, and Acidithiobacillus ferrooxidans [ 31 ]. There is a good understanding of the exact role of microbes in biomining, thanks to today's sophisticated instrumentation that can examine materials at the atomic level. Given the fact that many microbes float freely in the solution around the minerals, many microbes attach to the mineral particles forming a biofilm [ 31 ]. The microbes, whether they are freely floating or whether they are in the biofilm, continuously devour their food sources—iron (chemically represented as Fe 2+ ) and sulphur. The product of the microbial conversion of iron is “ferric iron,” chemically represented as “Fe 3+ ”. According to Brierley [ 32 ], ferric iron is a powerful oxidizing agent, corroding metal sulphide minerals (e.g., pyrite arsenopyrite, chalcocite, and sphalerite) and degrading them into dissolved melts, such as copper, zinc, and more iron, the latter being the food source for the microbes. The sulphide portion of the mineral is converted by the microbes to sulphuric acid.

Uranium occurs in oxidation states ranging from U (III) to U (VI), with the most stable species, U (VI) and U (IV), existing in the environment [ 34 ]. U (VI) is predominant in the oxic surface waters, and UO 2 2+ (uranyl) always forms stable, soluble complexes with ligands such as carbonate, phosphate, and humic substances [ 34 ]. In natural waters the solubility of U (VI) usually increases several orders of magnitude at higher pH values, due to complexation with carbonate or bicarbonate. By contrast, U (IV) is commonly found in the anoxic conditions and is present primarily as an insoluble uranite (UO 2 ). Therefore, reduction of the soluble uranyl to the insoluble uranite seems to be an effective means to immobilize uranium in the anoxic environment to decrease the potential release of the mobile species [ 34 ].

More research interests in the bioreduction of U (VI) are demonstrated in the dissimilatory metal-reducing bacteria (DMRB) under anaerobic conditions [ 34 ]. Lovley et al. [ 35 ] first demonstrated the occurrence of dissimulatory U (VI) reduction by the Fe (III) reducing bacteria Geobacter metallireducens and Alteromonas putrifaciens (later, Shewanella putrefacians ), which could conserve energy for anaerobic growth via the reduction of U (VI). Soluble U (VI) is more readily reduced to U (IV) by G. metallireducens and other Fe (III) reducing microorganisms than are insoluble Fe (III) oxides, and once produced, U (IV) can be reoxidized to U (VI) with the reduction of Fe (III) to Fe (II) [ 36 ].

10.2.2. Microbial Gold Mining

In some precious-metal deposits gold [ 32 ] occurs as micrometer-sized particles that are occluded, or locked, within sulphide minerals, principally pyrite (an iron sulphide mineral) and arsenopyrite (an arsenic containing iron sulphide mineral). According to Brierley [ 32 ], to effectively recover the precious metals, the sulfides must be degraded (oxidized) to expose the precious metals. Once the sulphides are sufficiently degraded to expose the gold and silver, a dilute solution of cyanide is used to dissolve the precious metals. If the occluded gold and silver [ 32 ] are not exposed by breaking down the sulphide minerals, the cyanide cannot help in the release of the metals and recovery will be low. The ferric iron that is produced by the microorganism is the chemical agent that breaks down (oxidizes) the sulfide mineral. The microorganisms can be thought of as the manufacturing facility for producing the ferric iron. Microorganisms in the ore are destroyed by lime. Cyanide leaching can be accomplished in another heap or the oxidized and lime-conditioned ore can be ground and cyanide leached in a mill. The residue slurry is rinsed with fresh water, neutralized with lime, subjected to solid/liquid separation, and the solid residue is cyanide leaching to extract the gold. Gold recoveries are in the 95–98% range.

Advantages of biomining [ 32 ] using organisms include the following.

• Biomining microorganisms do not need to be genetically modified; they are used in their naturally occurring form.
• Unlike humans, animals, and plants, microorganisms reproduce by doubling; that is, when there is abundant food (iron and sulfur) for biomining microbes and optimal conditions (sufficient oxygen, carbon dioxide and a sulfuric acid environment), a microbe will simply divide. Thus, in heap of minerals biooxidation for pretreating gold ores, there are about one million microbes per gram of ore.
• High altitudes have no effect on the biomining microorganisms. However, additional air must be supplied to give the organisms an optimal performance.
• Biomining using microorganisms does not produce dangerous waste products. Base metals, for example, zinc and copper are recycled and neutralized with lime/limestone.
• The biomining microbes cannot escape from the heap or bioreactor to cause environmental problems. These microbes exist in the environment only where conditions are suitable (i.e., sources of iron and sulfur are oxidized, air and a sulfuric acid environment).

Biomining as a biotool has not been explored in Nigeria. Though Nigeria has many solid minerals in different states of the country, some of the minerals are tin (found in Plateau, Nassarawa, Kaduna, Bauchi and Gombe states), gold (found in Oyo, Osun and Ondo states), copper (Edo and Benue states), tantalite (Gombe, Plateau, Kaduna, and Nasarawa states), and uranium (Bauchi state) among others. The procedures, equipments, nonawareness/interest by government as well as competition with the physicochemical methods of extraction of these minerals are the greatest limitation in the exploitation and usage of this biotool in mining of minerals from their ores. Providing information to Nigeria scientists, ministries and government agencies is the solution to this limitation. Thus, the essence of the suggestion here is to create awareness in this regard.

10.3. Biomonitoring

In a broad sense, biological monitoring involve any component that makes use of living organisms, whole or part as well as biological systems to detect any harmful, toxic, or deleterious change in the environment. There are various components employed in biomonitoring of contaminants in the environment. They include biomarkers (biological markers), biosensors, and many others.

Biomonitoring or biological monitoring is a promising, reliable means of quantifying the negative effect of an environmental contaminant.

Biological Markers —

A biomarker is an organism or part of it, which is used in soliciting the possible harmful effect of a pollutant on the environment or the biota [ 37 ]. Biological markers (biomarkers) are measurement in any biological specimen that will elucidate the relationship between exposure and effect such that adverse effects could be prevented [ 38 ]. The use of chlorophyll production in Zea mays to estimate deleterious effect of crude oil contaminants on soils is a typical plant biomarker of crude oil pollution [ 11 ]. When a contaminant interacts with an organism, substances like enzymes are generated as a response. Thus, measuring such substances in fluids and tissue can provide an indication or “marker” of contaminant exposure and biological effects resulting from the exposure. The term biomarker includes any such measurement that indicates an interaction between an environmental hazard and biological system [ 39 ]. It should be instituted whenever a waste discharge has a possible significant harm on the receiving ecosystem. It is preferred to chemical monitoring because the latter does not take into account factors of biological significance such as combined effects of the contaminants on DNA, protein, or membrane. Onwurah et al. [ 37 ] stated that some of the advantages of biomonitoring include the provision of natural integrating functions in dynamic media such as water and air, possible bioaccumulation of pollutant from 10 3 to 10 6 over the ambient value, and/or providing early warning signal to the human population over an impending danger due to a toxic substance. Microorganisms can be used as an indicator organism for toxicity assay or in risk assessment. Tests performed with bacteria are considered to be most reproducible, sensitive, simple, economic, and rapid [ 40 ] ( Table 3 ).

Biomarkers and their applications

10.4. Biosensor

A biosensor is an analytical device consisting of a biocatalyst (enzyme, cell, or tissue) and a transducer, which can convert a biological or biochemical signal or response into a quantifiable electrical signal [ 46 ]. A biosensor could be divided into two component analytical devices comprising of a biological recognition element that outputs a measurable signal to an interfaced transducer [ 24 ]. Biorecognition typically relies on enzymes, whole cells, antibodies, or nucleic acids, whereas signal transduction exploits electrochemical (amperometric, chronoamperometric, potentiometric, field-effect transistors, conductometric, capacitative), optical (absorbance, reflectance, luminescence, chemiluminescence, bioluminescence, fluorescence, refractive index, light scattering), piezoelectric (mass sensitive quartz crystal microbalance), magnetic, or thermal (thermistor, pyroelectric) interfaces [ 24 ]. The biocatalyst component of most biosensors is immobilized on to a membrane or within a gel, such that the biocatalyst is held in intimate contact with the transducer and may be reused. Biosensors are already of major commercial importance, and their significance is likely to increase as the technology develops [ 46 ]. Biosensors are still emerging biotechnology for the future in environmental biomonitoring since they have specific limitations. Biosensors on a general sense are often employed for continuous monitoring of environmental contamination or as bioremediation process monitoring and biocontrol tools to provide informational data on what contaminants are present, where they are located, and a very sensitive and accurate evaluation of their concentrations in terms of bioavailability. Ripp et al. [ 24 ] explained that bioavailability measurements are central to environmental monitoring as well as risk assessment because they indicate the biological effect of the chemical, whether toxic, cytotoxic, genotoxic, mutagenic, carcinogenic, or endocrine disrupting, rather than mere chemical presence as is achieved with analytical instruments. As the name suggests they are biological instruments that detest and signal the presence of harmful contaminants in the environment. There are different types based on the biological components on which their sensitivities are based ( Figure 4 ). Some of them, though not exhaustive are the following.

Anatomy of a Biosensor. The interaction between the target analyte and the biorecognition element creates a signalling event detectable by the interfaced transducer element. Modified from source: Ripp et al. [ 24 ].

10.4.1. Enzyme-Based Biosensors

Leyland Clark in the 1960s used an enzyme biosensor which consists of glucose oxidase enzyme immobilized on an oxygen electrode for blood glucose sensing. This historical application of enzyme-based biosensor has found a world-wide lucrative application in medical diagnosis. Nevertheless, enzyme-based biosensor gradually gained application in environmental monitoring. According to Ripp et al. [ 24 ], enzymes act as organic catalysts, mediating the reactions that convert substrate into product. Since enzymes are highly specific for their particular substrate, the simplest and most selective enzyme-based biosensors merely monitor enzyme activity directly in the presence of the substrate. A novel example of biosensors of enzyme origin which has found application in the environment is the sulfur/sulfate-reducing bacterial cytochrome C 3 reductases that reduce heavy metals. Michel et al. [ 47 ] immobilized cytochrome C 3 on a glassy carbon electrode and monitored its redox activity amperometrically in the presence of chromate [Cr (IV)] with fair sensitivity (lower detection limit of 0.2 mg/L) and rapid response (several minutes) ( Figure 5 ).

Enzymatic biosensor using cytochrome C 3 as the recognition element. Upon exposure to chromate [Cr (VI)], electrode-immobilized cytochrome C 3   reduces Cr (VI) to Cr (III). The current produced by the electrochemical regeneration of reduced cytochrome C 3 is proportional to the amount of oxidized cytochrome C 3 and, therefore, the Cr (IV) concentration. Ripp et al. [ 24 ].

When tested under simulated groundwater conditions, the biosensors reacted with several other metal species, albeit at lower sensitivities, and were affected by environmental variables such as pH, temperature, and dissolved oxygen. Similarly operated enzyme-based biosensors for ground water contaminant perchlorate using perchlorate reductase as the reduction enzyme (detection limit of 10  μ g /L) [ 48 ], organophosphate pesticides using parathion hydrolase or organophosphorus hydrolase as recognition enzymes (detection down to low μ M concentrations) [ 49 ], and environmental estrogens using tyrosinase as the recorgnition enzyme (detection down to 1   μ M) [ 50 ] have also been designed.

Another type of enzyme biosensor relies on enzyme activation upon interaction with the target of interest. Heavy metals, for example, in the form of cofactors-inorganic ions that binds to and activate the enzyme can be detected based on this integral association. Metalloenzymes such as alkaline phosphatase, ascorbate oxidase, glutamine synthetase, and carbonic anhydrase require association of a metal ion cofactor with their active sites for catalytic activity and can thus be used as recognition element for heavy metal [ 24 ]. Alkaline phosphatase, for example, can be applied in this regard as a biosensor for zinc [Zn (II)] or ascorbate oxidase for biosensing copper (II) with detection limits down to very low part-per-billion levels [ 51 ]. Various immobilization techniques are adopted in the attachment of the enzyme to the transducing element [ 52 ]; they include adsorption, covalent attachment, entrapment in polymeric matrices such as sol-gels or Langmuir-Blodgett films, or direct cross-linking using polymer networks or antibody/enzyme conjugates. Immobilization provides the biosensor longevity and with recent integration of redox active carbon-based nanomaterials (nanofibers, nanotubes, nanowires, and nanoparticles) as transducers and their unique ability to interact with biological material, a promising advancement in enzyme biosensor design and sensitivity is in sight.

Optical transducers (absorption, reflectance, luminescence, chemiluminescence, evanescent wave, surface plasma resonance) are also commonly employed in enzyme-based biosensor [ 24 ]. This can be as simple as optically registering a pH change using a pH reactive dye; for example, bromocresol purple can be immobilized with an acetylcholinesterase-based biosensor to monitor pH changes related to this enzyme's activity upon exposure to pesticides. Acetylcholinesterase hydrolysis releases protons (H + ), resulting in a decrease in pH, which in turn instigates a decrease in the absorption spectra of bromocresol purple [ 24 ].

Andreou et al. [ 53 ] incorporated such a biosensor successfully on the distal end of a fibre optic cable for facile interrogation of water samples for pesticide residue. A great application of optical biosensor is in the Luminol, widely used as an electrochemiluminescent indicator. It reacts with the acetylcholinesterase/choline oxidase hydrogen peroxide by-product to yield luminescent light signals that have also been used to quantify pesticide concentrations.

10.4.2. Antibody-Based Biosensors (Immunosensors)

These types of biosensors make use of antibodies as recognition elements (immunosensors). They are used widely as environmental monitors because antibodies are highly specific, versatile, and bind stably and strongly to target analytes (antigens) [ 24 ]. Antibodies can be highly effective detectors for environmental contaminants, and advancements in techniques such as phage display for the preparation and selection of recombinant antibodies with novel binding properties assures their continued environmental application. Perhaps the best introduction to antibody-based biosensing is the Automated Water Analyzer Computer Supported System (AWACSS) environmental monitoring system developed for remote, unattended, and continuous detection of organic pollutants for water quality control [ 54 ]. AWACSS uses an optical evanescent wave transducer and fluorescently labelled polyclonal antibodies for multiplexed detection of targeted groups of contaminants, including endocrine disruptors, pesticides, industrial chemicals, pharmaceuticals, and other priority pollutants, without requisite sample preprocessing. Antibody binding to a target sample analyte occurs in a short 5-minute preincubation step, followed by microfluidic pumping of the sample over the transducer element, which consists of an optical waveguide chip impregnated with 32 separate wells of immobilized antigen derivatives [ 24 ]. As the antibody/analyte complexes flow through these wells, only antibodies with free binding sites can attach to the well surface (in what is referred to as a binding inhibition assay). Thus, antibodies with both of their binding sites bound with analyte will not attach to the surface and will pass through the detector. A semiconductor laser then excites the fluorophore label of bound antibodies, allowing for their quantification, with high fluorescence signals indicating high analyte concentrations. A fibre optic array tied to each well permits separation and identification of signals by the well, thereby yielding a simultaneous measurement of up to 32 different sample contaminants. The instrument has been used for groundwater, wastewater, surface water, and sediment sample testing with detection limits for most analytes in the ng/L range within assay times of approximately 18 minutes [ 24 ]. Another design by Glass et al. [ 55 ], similar to the above but less refined benchtop flow-through immunosensor (KinExA) was demonstrated to detect analytes successively based on a replaceable flow cell containing fluorescently labeled antibody. Their time of assay was approximately 26 minutes, with detection limits at picomolar concentrations.

Although not as elaborate as the AWACSS, a multitude of other antibody-based biosensors have been applied as environmental monitors, traditionally serving as biosensors for pesticides and herbicides, but their target analytes have broadened considerably over the past several years to include heavy metals, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), explosives (TNT and RDX), phenols, toxins such as microcystin, pharmaceutical compounds, and endocrine disruptors [ 56 ].

10.4.3. DNA-Based Biosensors

The principle underlying the DNA-based biosensor is the ability of a transducer to monitor a change in the nucleic acid's structure occurring after exposure to a target chemical. These structural changes are brought on either by the mutagenic nature of the chemical, resulting in mutations, intercalations, and/or strand breaks, or by the chemical's ability to covalently or noncovalently attach to the nucleic acid [ 24 ]. Immobilizing the nucleic acid as a recognition layer on the transducer surface forms the biosensor, and detection of the chemically induced nucleic acid conformational change is then typically achieved electrochemically (i.e., a change in the current) or less so through optical or other means [ 57 ].

Nucleic acid biosensors are generally nonselective and provide an overall indication of a potentially harmful (genotoxic, carcinogenic, cytotoxic) chemical or chemical mix in the test environment and, depending on the biosensor format, an estimate of concentration. Bagni et al. [ 58 ] illustrated a conventional DNA biosensor which was used to screen soil samples for genotoxic compounds, using benzene, naphthalene, and anthracene derivatives as model targets. Double-stranded DNA was immobilized on a single-use disposable screen-printed electrochemical cell operating off a handheld battery-powered potentiostat [ 59 ]. A 10  μ L drop of a preprocessed and preextracted contaminated soil sample was placed onto the working electrode for 2 minutes, and resulting electrochemical scans, based on the chemical's propensity to oxidize DNA guanine residues, were measured. The magnitude of these “guanine peaks” in relation to a reference electrode was linearly related to their concentration in solution (i.e., the higher the concentration of the target chemical, the more the damage imposed on the DNA, and the lower the electrochemical measurement of the oxidation signal). In a very discrete application of this DNA biosensor, the authors also applied it to the detection of this DNA biosensor and also to the detection of PAHs in fish bile, using the accumulation of PAH compounds in live fish to monitor for water contamination events [ 60 ].

Nucleic acid can be manipulated similarly to create target specific aptamers using a process called SELEX (systematic evolution of ligands by exponential enrichment) [ 24 ]. By iteratively incubating nucleic acid with the desired target, one can select for oligonucleotide sequences (or aptamers) with the greatest affinity for the target. Kim et al. [ 61 ] used SELEX to create an aptamer specific for 17  β -estradiol and used it in an electrochemical biosensor (or aptasensor) to achieve detection of this important endocrine disruptor at levels as low as 0.1 nM. Predominant aptasensor development and application is in the clinical fields, but it is slowly and inevitably encroaching upon environmental sensing. An aptasensor for the cyanobacterial toxin microcystin (lower dertection limit of 50 μ g / mL) [ 62 ] and another for zinc based on fluorophore beacon (lower detection limit of 5  μ M) [ 63 ] have been reported.

Hydrazine and aromatic amine compounds in fresh and groundwater, hydroxyl radicals in uranium mine drainage waters, herbicides such as atrazine, general toxicity events in wastewater, industrially contaminated soils, and various other environmental sources have all been screened using DNA biosensors [ 24 ].

Metals are also relevant detection targets, due to their various affinities for nucleic acid. Lead, Cadmium, Nickel, Arsenic, Copper, Iron, Chromium, and others have been detected through DNA biosensing, incorporating both single- and double-stranded DNA as the sensing element, but again, nonselectivity [ 24 ]. Selectivity, though, has been demonstrated by several groups using deoxyribozymes (DNAzymes) or ribozymes (RNAzymes). These engineered catalytic oligonucleotides can mediate nucleic acid cleavages or ligation, phosphorylation, or other reactions. For example, DNAzyme biosensor for lead uses a single-stranded DNAzyme absorbed to a gold electrode [ 64 ]. The DNAzyme incorporates a methylene blue tag at concentrations as low as 62 ppb; the DNAzyme strand is cleaved, allowing the methylene blue tag to approach the transducer and transfer electrons, thereby instigating an electrochemical signal [ 24 ]. However, the rapidity (only a few minutes to detect but sample processing is often necessary), sensitivity (typically down to low part-per-billion levels), ease of use, and cost-effectiveness screen environmental sites for toxic chemical intrusions or monitoring operational endpoints of bioremediation efforts. A calorimetric DNAzyme-based biosensor for lead has also been demonstrated [ 65 ].

10.4.4. Biomimetics, BioMEMs, and Other Emerging Biosensor Technologies

The future of biosensors is clearly in the emergent technologies of Biomimetics and BioMEMs. BioMEMs (biological microelectromechanical systems) are an assortment of biomicro, bioanotechnological, and microfluidic interfaces that form lab-on-a-chip, biochip, or micrototal analysis system ( μ  TAS) biosensors [ 24 ]. Their objectives are toward miniaturization, portability, redundancy, and a reduction in sample size, time of response, and cost. The majority of these biosensors serves biomedical rather than environmental causes, but they are slowly and inevitably being adapted for the environmental monitoring community. BioMEMs most often utilize optical transducers interfaced with enzyme, whole-cell, antibody, or nucleic acid-type receptors. Several recent examples should illustrate their various design and performance characteristics. Yakovleva et al. [ 66 ] developed a microfluidic immunosensor flow cell for the detection of atrazine in surface water. Chemiluminescently labelled antibodies directed against atrazine were combined with artificially contaminated river water and microfluidically pumped at 40 to 50 μ L / minute through a 42-channel 13 mm × 3 mm silicon microchip containing a functionalized antibody affinity-capture surface [ 24 ]. Upon antibody/atrazine capture, a luminal substrate was added to mediate the chemiluminescent reaction which was monitored with a photomultiplier tube (PMT) suspended above the microchip. Islam et al. [ 67 ] have further improved this sensing strategy by essentially integrating the PMT directly on the microchip flow cell to create truly miniaturized biosensor referred to as a BBIC (bioluminescent bioreporter integrated circuit). This 1.5 mm × 1.5 mm CMOS microluminometer was designed to capture and process bioluminescent signals emanating from immobilized whole cell bioluminescent bioreporter bacteria [ 24 ]. The BBIC converts the bioluminescently derived photodiode current into a digital signal, the frequency of which is proportional to the concentration of pollutant to which the bioreporter has been exposed. In water artificially contaminated with salicylate as a model pollutant, the flow-through BBIC responded with 30 minutes to part-per-billion concentration [ 24 ].

BioMEMs also include microcantilever-based biosensors that translate a molecular recognition event into nanomechanical motion that is measured by induced bending in a microfabricated cantilever similar on a macroscale to identifying a person on a diving board based on the deflection of the diving board by their weight. Optical or piezoresistive transducers usually measure microcantilever deflections at nanometer-to-subnanometer ranges of motion, and due to their small size, several microcantilevers can be accommodated per transducer for multianalyte sensing. Alvarez et al. [ 68 ] immobilized antibodies to the pesticide DDT on a microcantilever and demonstrated real-time detection at nanomolar concentrations.

Biomimetics mimic (imitate) the attributes of naturally occurring biological materials to synthetically recreate or enhance their properties [ 24 ]. Molecularly imprinted polymers (MIPs) are one of the typical examples of Biomimetics application in biosensors which can deliver more robust, stable, and target-specific receptors. MIPs are essentially created by mixing the target analyte (or template) with a monomer. Resulting MIPs then serve as analyte-specific synthetic receptors (or artificial antibodies or enzymes) that can be associated with transducers to form sensors. Dickert et al. [ 69 ] synthesized MIP receptors for various PAH constituents, optically interrogated them with a fluorescent sensor, and demonstrated detection of individual PAHs such as pyrene down to ng/L concentrations in artificially contaminated drinking water. More recently, Xie et al. [ 70 ] molecularly imprinted the explosive 2,4,6-trinitrotoluene (TNT) onto the walls of silica nanotubes, thus implying a great future for MIP nanosensors which have faster response time and great sensitivity. Other environmentally relevant MIP sensors have been designed for various herbicides/pesticides (2,4-D, atrazine, phenylureas, CAT, DDT), aquatic toxins such as microcystin and demoic acid, and various heavy metals, with incorporation into a variety of optical, electrochemical, or piezoelectric transducer element [ 71 ].

Biosensors based on the use of whole animals or their organs represent a very unique mode of sensing. Insect antennas, for example, are covered with highly sensitive and naturally tuned receptors called sensilla that respond to chemical, physical, and mechanical signals via electrical nerve impulses. By immobilizing the antenna or even the entire insect on a transducer and measuring these induced electrical impulses (or electroantennograms), a biosensor materializes [ 24 ]. A multianalyte biosensor can be formed by adhering antennas from several different insects. The current targets for such biosensors are odourants such as those related to smoke (guaiacol and 1-octen) for early-warning fire detection or volatiles emanating from diseased plants, with detection limits in the part-per-billion range. Their parallel applications for sensing volatiles associated with environmental contaminants and even non-odour-related compounds are a potential future prospect [ 24 ]. Imagine a chip sensitive to a particular pollutant analyte attached to a fish, insects, or invertebrate and the organism released into the environment while the sensor is monitored with a computer. The possibility is as much as the imagination can go!

10.5. Biomass Fuel

Biomass is any plant or animal matter used to produce energy. Many plants and plant-derived materials can be used for energy production; the most common is wood. Other sources include food crops, grasses, agricultural residues, manure, and methane from landfills [ 72 ]. The main driving forces for adoption of biomass fuel and its encouragement is mainly due to the more efficient bioprocesses and bioproducts which are cost savings and improved product quality/performance. Environmental consideration of the quick degradation of by-products of biomass fuel is a major consideration in the development of this biotool.

The state of Texas in the United States of America is known to be an agricultural state that has adopted biomass energy production. Crops used to produce biomass energy include cotton, corn, and some soybeans—all grown in Texas [ 73 ]. In the US, the primary biomass fuels are wood, biofuels, and various waste products. Biofuels include alcohols, synfuel, and bio-diesel, a fuel made from grain and animal fats. Waste consists of municipal solid waste, landfill gas, agricultural by-products, and other material. Most biomass energy used in the US—65 percent—comes from wood [ 74 ]. Another 23 percent of biomass energy used comes from biofuels. while the remaining 12 percent comes from waste energy. Energy generated from biomass is the nation's largest source of renewable energy, accounting for 48 percent of the total in 2006. The US consumed 3,277 trillion British thermal units (Btu) of biomass energy in 2006. The next largest source of renewable energy is hydroelectric power, with 2,889 trillion Btu consumed in 2006 [ 75 ].

While cattle manure has the most potential for power use, other forms of agricultural waste have significant possibilities, too. These include poultry litter, rice straw and husk, peanut shells, cotton gin trash, and corn stover. In fact, a recent report from the Houston Advanced Research Center estimated that Texas agricultural wastes have the potential to produce 418.9 megawatts of electricity, or enough to power over 250,000 homes, based on average Texas electricity use in 2006 [ 76 ].

Plant biomass can be processed and converted by fermentation and other processes into chemicals, fuels and materials that are renewable and result in no net emissions of greenhouse gases. Also, energy, such as waste heat, can be used efficiently. This approach is called industrial ecology [ 77 ].

In the US, ethanol made from corn currently accounts for the majority of biofuel consumption in the transportation sector. In the future, however, “lignocellulosic” biofuels made from crop residue, grasses, wood products, sorghum, “energy cane,” and agricultural waste are expected to supplement corn ethanol. These are commonly referred to as “cellulosic.” Public and private funding for new research in cellullosic fuels is increasing. Corn ethanol requires significant amounts of fertilizers, pesticides, energy and water to grow, cellulosic biofuel production promises to be much more efficient. The production of cellulosic ethanol and other biofuels is expected to give significant increase in yield of fertilizers, pesticides and energy production. Cellulosic biofuel production promises to be much more efficient in economic usage of agricultural products.

Biologically derived products (bioproducts) are generally less toxic and less persistent than their petrochemical counterparts. Group of companies can mimic the cooperative action of organisms in natural ecosystems by clustering around the processing of a feedstock such as a biomass so the by-product of one is the starting material for another. The ability to evolve bioprocesses and bioproduction systems allows for major improvements in both economic and environmental performance. This permits a manufacturing facility to increase its profitability and capacity while maintaining or even reducing its environmental footprint [ 77 ].

In Nigeria, lots of projects are ongoing in the area of biofuel. Dependence on crude oil as a major economic stay for the country has also had its toll on other sectors such as agriculture. Despite this, Nigeria is yet to develop the refining sector of the crude oil exploration. Nigeria currently imports refined petroleum, diesel, ethanol, and kerosene from countries around the world. This has not adversely affected the price of the commodity in the country. Its negative effect is slowly being realized. As a result, it is wise to quickly look at alternative sources of energy so as to avoid the short fall of overdependence on nonrenewable sources such as the petroleum industry so as to cushion the gradual but sure effect of this activity in the economy of the country. The government is also gradually looking at ways to refine the crude oil by revitalizing the refineries and making them functional. Despite all these measures, biofuel still holds the answer to the solution of the problems in the oil sector as long as the environment is concerned. Also the future portends that better industrial management practices will preferentially make use of agro-based sources in all production process so as to put the environment first in all production practices. Two of such ongoing research in this biotechnological tool process involve: Alkali-catalysed Laboratory Production and Testing of Biodiesel Fuel from Nigerian Palm Kernel Oil by Alamu et al. [ 78 ] and BioDiesel Nigeria (BDN), a company that will provide jobs for Nigerians by farming Jatropha seeds. The company had proposed on providing employments for those living in poverty in the cities by relocating them to Jatropha farms in the north. There they will be trained to farm Jatropha trees and be paid a decent wage. Tables ​ Tables4 4 and ​ and5 5 show recorded extent of Biofuel efforts in Nigeria.

Bioethanol plants in Nigeria.

Proposed plants.

NA: not available.

Source: Agbola et al. [ 79 ].

Highina et al. [ 80 ] stated that biofuel industry in Nigeria is still at its infancy, even though policy guidelines are available at the NNPC for the development of the industry, few most ground breaking achievements have been made. In 2007 the Kaduna State Government of Nigeria set up a pilot plant for bioethanol production to demonstrate the technology and its viability using local design and materials. Ahmadu Bello University, Zaria, Kaduna State Nigeria, also established a pilot plant for biodiesel production Bugaje and Mohammed [ 81 ]. Other efforts elsewhere in the country have been limited to bench-scale production of bioethanol and biodiesel from a number of feedstock. There is need to move further from this and scale up for commercial production. Ethanol is produced from fermentation of sugars and biodiesel from the reaction of plant or animal oils with an alcohol, for which methanol is universally used in commercial application today [ 80 ].

10.6. Aquaculture Treatment/Management

Catfish cultivation has assumed a commercial dimension world-wide. With the adoption of this agricultural wealth creation technique, and the uniqueness of its good protein content, it has been accepted as special delicacy among the wealthy especially in Nigeria. Most families in south-eastern and south-western Nigeria cultivate catfish on medium to commercial scale as a financial supplement for regular job. Most individual, have also engaged themselves as full-time catfish farmers in different level of this aquaculture business. Thus, some are into catfish feed production, breeding (reproduction) as well as total development and marketing of this agricultural product. Apart from the fact that there are various feeds available in the market for cultivation of catfish, various feed supplements from poultry waste to domestic food remnants as well as vegetables, nuts, fruit peels, and so forth are also integrated in the production of this fast growing aquaculture practice. In line with this encouraging agricultural venture is the attendant problem of effluent generation which can adversely affect the aesthetic environments.

Catfish pond effluent quality varies from pond to pond and from season to season. Effluent quality is usually poorest (highest concentrations of solids, organic matter, total phosphorus, and total nitrogen) in the summer when fish feeding rates and water temperatures are highest. Catfish pond effluents generally have higher concentrations of nutrients and organic matter than natural stream waters but much lower concentrations than municipal, and industrial waste water. It appears that catfish pond effluents are most likely to exceed regulatory limits for suspended solids and total phosphorus. Other measured water qualities sometimes are used for treating agricultural, municipal and industrial waste water.

According to SRAC [ 82 ] report, there are various biological techniques adopted, which are cost efficient and eco-friendly in the treatment of aquaculture effluents. Some of such aquaculture biotechniques include the following.

Construction of Wetlands Adjacent to Ponds —

Wetlands are inexpensive to build and operate, and it also eliminates the need for chemical treatment of wastewater. They also contribute stability to local hydrologic processes and are excellent wild-life habitats.

The disadvantage of wetland for treating aquaculture pond wastes is the large amount of space necessary to provide an adequate hydraulic residence time. Therefore, it will probably be necessary to integrate wetland treatment of effluents with other pond effluent management procedures to reduce the area of wetland needed. For example, a wetland centrally located on a farm, or connected to an integrated drainage system, would save on construction costs and use land efficiently. Such a system would also allow a wetland to be used to treat the overflow coming from ponds after rainfall. Pond draining could be staged so that only one pond is being drained at a time, allowing one wetland to serve numerous ponds. Effluent from a constructed wetland could even be pumped back into ponds and reused if needed.

Wetlands act as biological filters to remove pollutants from water, and natural or constructed wetland exposes the solid, semisolid waste particles for fast degradation by microorganism. The water if properly channeled can be reused, and the dried solid component of the effluent when properly treated can be used as organic fertilizer in crop production [ 82 ].

10.6.1. Treating Pond Effluents Using Grass Filter Strips

Draining effluents over grass strips filters solids from animal waste. This system may be useful for filtering catfish pond effluent. Common and coastal Bermuda, dallies, and Bahia are recommended grasses for warm climates; fescue, reed canary, and rye grasses are recommended for cool climates. Grass filter strips are highly effective in reducing the concentrations of suspended solids, biochemical oxygen demand, and ammonia, but not efficient in removing algae.

Concentrations of suspended solids, organic matter, and total nitrogen in catfish pond effluents were reduced by applying the effluent to well-established strips of either Bahia or Bermuda grass. This filtering technique was relatively easy and inexpensive and may have application if the filtered effluent is to be reused for fish production to conserve groundwater. It could also be used to treat effluent before discharging it to receiving water [ 82 ].

10.6.2. Management Practices to Reduce the Impact of Aquaculture Effluents on the Environment

(a) conservative water management practices —.

This involves two major techniques, namely,

• reusing water for Multiple Fish Crops
• reducing overflow after rains by keeping pond water level below the pond drain.

Reusing Water for Multiple Fish Crops —

The concentration of the substance in the effluent is dependent on the volume of water discharged as well as the mass of nutrients (organic matter) present in the ponds according to the aquacultural feeding history. Thus, reducing the concentration of potential pollutants in pond effluents is difficult, but it is relatively easy to control discharge volume. The most obvious procedure for reducing the volume of effluents from channels catfish ponds is to harvest the fish without draining the ponds. However, this practice works only if water quality does not deteriorate as the water is reused.

SRAC [ 82 ] report established that a comparison of annually drained and undrained catfish ponds showed little difference in water quality and no difference in fish production. Natural processes, such as nutrient uptake by bottom soils, microbial decomposition of organic matter, denitrification and sedimentation, continually remove potential pollutants from pond water. Operating ponds without draining makes better use of the waste assimilation capacity of ponds and saves significant amounts of water as well as reducing overall effluent volume.

Reducing Overflow after Rains by Keeping Pond Water Level below the Pond Drain —

Seasonal changes in overflow volume affected the amount of waste discharged more than seasonal changes in effluent quality [ 82 ]. So, reducing overflow volume can have a dramatic impact on mass discharge of nutrients and organic matter from catfish ponds. By keeping the pond water level below the level of the drain, rainfall is captured rather than allowed to overflow and annual waste discharge is reduced by 50 to 100% depending on the weather. Specifically, waste discharges are normally greatest in winter when overflow volume are highest and not in summer when waste concentrations are highest. A study was conducted in which effluents from Georgia catfish ponds were used to determine the production of soybean irrigated with such effluents.

(b) Using Effluents for Irrigation of Soybeans —

Although water discharged from aquaculture ponds is often viewed simply as a waste product, it still has value and its reuse may have multiple benefits. If ponds are located near terrestrial crops that require irrigation, pond discharge can be used for irrigation water. That use will reduce waste discharge and benefit the crop. The SRAC [ 82 ] report has it that the total nitrogen available for crops varied from 0.9 to 1.2 kg/ha from each centimeter of water applied. Assuming average irrigation is 30 cm, then available nitrogen ranged from 27 to 36 kg/ha, a significant portion of the nitrogen requirement of many agronomic crops. Although the average soybean yield was 3.6 metric tons/ha, double the average yield in Georgia, the increased yield was the result of irrigation alone and not the nutrients in the irrigation water. Although the nutrient content of pond effluents may be too low to affect crop production, effluent water not useful for catfish cultivation can find application for irrigation of crops and thus reduce discharge volume. Rice irrigation with effluents of aquaculture has also been suggested.

It is important to note that even though catfish farming is the major aquaculture, there are also other aqua cultural practices like cultivation (breeding) of crawfish, shrimps, and other fresh water organisms.

The summary of the SRAC [ 82 ] publication is an examination of the impact of aquaculture pond effluent on the environment and how the use of cost reduction and yet simple management practices can help to control pollution of the environment with aquaculture effluents. These simple management practices do not require extra expense or labour. It is advocated therefore that all aquaculturists should strive to reduce the impact of their activities on the environment by adhering to the following guidelines (aquaculture management practices).

• Use high-quality feeds and efficient feeding practices. Feeds are the origin of all pollutants in catfish pond effluents.
• Provide adequate aeration and circulation of pond water. Maintaining good dissolved oxygen levels enhances the appetite of fish and encourages good feeding conversion. Oxygen availability at bottom of ponds improves degradation of organic matter and reduces the amount of organic matter in effluent.
• Minimize water exchange. Routine water exchange is of questionable value as a water quality management procedure and greatly increases effluent volume.
• Operate ponds for several years without draining. Reusing water for multiple fish crops is one of the best methods of reducing waste discharge from ponds.
• Capture rainfall to reduce pond overflow also reduces the need for pumped water to maintain pond water levels.
• Allow solids to settle before discharging water. After sieving ponds partially drained for fish harvest, hold remaining water for 2 to 3 days to allow solids settle. Better still dose not discharge this last portion of water.
• Reuse water that is drained from ponds. Instead of draining ponds for fish harvest, water can be pumped to adjacent ponds and reused in the same or other ponds.
• Treat effluents by using constructed wetlands.
• Use effluents to irrigate terrestrial crops. Under certain conditions, the water discharged from ponds may have value as irrigation water for crops.

10.7. Biocatalysts

By using well-established tools from metabolic engineering [ 83 ] and biochemistry [ 84 ], efforts have been made on engineering microbes to function as “designer biocatalysis,” in which certain desirable traits are brought together with the aim of optimizing the rate and specificity of biodegradation. Therefore, enzymes extracted from naturally occurring microorganisms, plant and animals can be used biologically to catalyse chemical reactions with high efficiency and specificity. Compared to conventional chemical processes, biocatalytic processes usually consume less energy, produce less waste, and use less organic solvents (that then require treatment and disposal) [ 77 ].

Microbial industrial production of enzyme involves a lot of aerobic steps within a submerged culture in a stirred tank reactor. The enzyme biochemistry is driven by transcription, translation, and molecular mass, number of polypeptide chains, isoelectric point, and degree of glycosylation, such as a saccharide's reaction with a hydroxyl or amino group to form a glycoside [ 20 ]. The selection of microbes as candidates for fermentation depends on process characteristics (such as viscosity or recoverability), legal approval of use, and the state of knowledge about the selected organism. Sugars comprise the principal feedstock (i.e., production process strictly biological) for microbial processes (carbon and energy sources) [ 20 ]. Feedstocks include molasses, unrefined sugar, and sulfite liquor from cellulose production plants, hydrolysates of wood and starch, or fruit juices, such as the grape juice used in wine making processes. Thus, these raw sources contain other compounds beside sugars. This can be beneficial, because vegetative materials invariably contain nitrogen, phosphorus, and potassium, important nutrients to maintain microbial growth and metabolism [ 20 ]. For the purer feed stocks, the nutrients are added to the reactor as inorganic compounds such as ammonium compounds, phosphate, and potassium chloride. Organic supplements include meal, fish meal, cotton seed, low-quality protein materials such as casein or its hydrolysates, millet, stillage, and corn steep liquor. In addition, these chemically complicated mixtures must contain micronutrients, that is trace elements and growth promoters, which are limiting factors. In general, the raw materials are dissolved or suspended in water, and then the medium is heated, filtered, and sterilized. For downstream processing (harvest, concentration, and purification) or for analytical assays during the process, additional pretreatment of the raw material can reduce unwanted side reactions [ 20 ] ( Figure 6 ).

Steps in industrial fermentation (enzyme production). Source: [ 25 ].

By imitating natural selection and evolution, the performance of naturally occurring enzymes can be improved. Enzymes can rapidly be “evolved” (this technique is called “molecular evolution”) through mutation or genetic engineering and selected using high-throughput screening to catalyse specific chemical reactions and to optimize their performance under certain conditions such as elevated temperature [ 77 ].

11. Nigeria Situation

In research institutions and universities, biocatalysis have been applied in laboratory-scale experiments and primary investigation of product synthesis. Most of the detergents in the country are incorporated with various enzymes. The most significant areas of application of biocatalysis as a biotechnological tool in Nigeria are in the areas of yoghurt production by many local industries, confectionaries and bread production industries, local fermented special seasonings (Ogiri and Okpei) for soup making in the eastern part of Nigeria, produced by boiling melon seed, castor oil seeds and exposing them to microorganisms in the environment for fermentation followed by milling and packaging for consumption. Others involve fermentation of cassava for food and brewing of alcoholic drinks by pilot commercial industrial production in organizations such as Nigerian Breweries, Nigerian Distilleries, Guinness Nigeria Plc, and many other brewing industries which spread across various states in the country ( Table 6 ).

Environmental friendly application of enzymes.

Source: adapted from: [ 93 ].

Enzyme biotechnologies can be visualized as sets of biological reactions occurring at various scales in the environment. The activities of enzyme in reactions can lead to desirable results, such as the chemical transformation and ultimate degradation of toxic substances into harmless compounds. Biological reactions may also lead to undesirable results, such as the introduction of genetically modified organisms to an ecosystem or the generation of toxic chemicals. Here enzymes are modified to do the clearing process of these toxins.

12. Conclusion

At the backdrop of the need to meet certain challenges that affect development, thus the much needed change to bring about these developments informed the decision by the United Nation to elaborate on key agenda which most nations are expected to adhere to in order to achieve certain goals known as Millennium Development Goals (MDGs). The attainment of these goals, aiming at ensuring that participating countries, provide basic good things of life for their citizens.

The United Nations (UN) Secretary General's Special Adviser on the MDGs, Jeffrey D. Sachs, visited Nigeria recently for assessment of progress in indicators of whether Nigeria is on the part of attaining these (MDGs) and he gave his verdict. In his word as reported by Anuforo [ 94 ] “One would say Nigeria is on the path, but not well on the path. The direction is positive. The institutional innovation is exciting. But the quantitative achievement is not sufficient. So, there really need to be acceleration between 2010 and 2015.”

On her part, the Senior Special Assistant to President Goodluck Jonathan on the MDGs, Mrs. Amina Az-Zubair, also gave perspective to what the Nigerian Government is doing to attain the 2007 to 2016 objectives. According to Agbaegbu [ 95 ], Az-Zubair said that the overarching objective of the countdown towards Nigerians achievement of the MDGs by 2015, on to safe water and sanitation have not improved significantly, and other environmental challenges such as erosion, coastal flooding, and climate change are growing. The MDG office reported, however, that the proportion of the population with access to safe drinking water dropped from 54 percent in 1990 to 49 percent in 2007. The proportion of the population with access to basic sanitation is said to have risen from 39 to 43 percent in the recent period. “With better implementation of the various policy frameworks and plans for water, sanitation, environment and slum upgrading, Nigeria will be on track to achieve this goal,” the MDG office report said. Even so, it noted that “planning and maintenance of water supply at local level is weak. Environmental pressures range from desertification in the North to flooding, rubbish heaps and soil erosion in the coastal and Niger Delta regions, requiring a nationally coherent but localized approach.”

Enduring sustenance of the environment must come from using the natural methods to remove the synthetic or deleterious activities of man in the environment. When a forest, for example, loses its trees it takes some time to regenerate but when it does it retains it natural beauty and improve the quality of other biota within it. When a lake is contaminated with pollutants, prevention of more pollution and exploitation of gases (aeration), encouragement of microbial activities restores the health of the lake. Thus, biotechnological tools are bioenvironmental technological practices aimed at encouraging without compromise with any other technology no matter how fast and efficient those other technologies could be in restoring the environment in such a way that is closer to nature if not totally natural.

The biotools enumerated by the authors are by no means exhaustive since environmental biotechnology is highly dynamic as well as futuristic. Those commonly practiced much elsewhere and very little if at all in Nigeria, which are being improved upon, are aquaculture treatment/management, biomonitoring, bioleaching, biocatalysis, biodetergent/biosolvent production, biofiltration, bioremediation, biomass fuel production, and so forth. Therefore, improvement in the quality of human, environment anywhere can be attained by applying any of the biotechnological tools suitable in such an environment. Nigeria has already adopted some such as bioremediation, biomonitoring, biofiltration, biofuel, and biocatalysis; however, their volume of their adoption is still very low. There is therefore need to adopt these biotools much and to look at ways of encouraging the local industries to adopt biotechnological tools in their production process so as to maintain the Nigerian environment.

Developing Nations should instead of despairing on the damaged environment due to refuse heaps, mining activities, industrial production, and so forth embrace these practice (biotools) so as to improve their environment as well as to maintain and prevent continuous degradation of the environment. The Niger Delta regions of Nigeria had experienced continuous defacing of the environment due to crude oil pollution. Other nations have also had battered environments (water, soil, air) due to numerous industrial activities. Finding alternative ways of production, favourable to the environment as well as eco-friendly means of dealing with waste will go a long way in sustaining the environment.

Acknowledgments

The Library section of Godfrey Okoye University, Enugu-Nigeria, is sincerely acknowledged and of worthy mention is Mrs. Mary Ellen Chijioke whose magnanimity is unequalled and who took time to personally search and provide basic environmental biotechnology textbooks which in no small measure helped in the success of this research. The Dean Faculty of Natural and Applied Sciences, Godfrey Okoye University, Professor Emmanuel Adinna, is sincerely appreciated for the constant reminder that “this work must be done.” The ebullient and amiable Vice Chancellor of Godfrey Okoye University, Enugu Nigeria, Most Rev. Fr. (Professor) Christian Anieke is most appreciated for his untiring struggles to make Godfrey Okoye -University the best in the world.

• © 2022

Biotechnological Innovations for Environmental Bioremediation

• Sudipti Arora 0 ,
• Ashwani Kumar 1 ,
• Shinjiro Ogita 2 ,
• Yuan -Yeu Yau 3

Department of Biotechnology, Dr. B. Lal Institute of Biotechnology, Jaipur, India

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Department of Botany, University of Rajasthan, Jaipur, India

Department of local resources and department of life sciences, prefectural university of hiroshima, hiroshima, japan, department of natural sciences, northeastern state university, broken arrow, usa.

Covers methodological developments of phytoremediation research

Explores the mechanisms in plants supporting degrade, take up, or tolerate the effects of pollutants

Provides multidisciplinary approach, methods and reviews to principles and practices of phytoremediation

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Table of contents (40 chapters)

Front matter, environmental remediation, ecosystem engineers: a sustainable catalyst for environmental remediation.

• Devanshi Sutaria, Sonika Saxena, Kamlesh R. Shah, Sudipti Arora

Microbial Nanobiotechnology in Environmental Pollution Management: Prospects and Challenges

• M. B. Alao, C. O. Bamigboye, E. A. Adebayo

Soil Microbiome: A Key Player in Conservation of Soil Health Under Changing Climatic Conditions

• Hiren K. Patel, Rishee K. Kalaria, Divyesh K. Vasava, Hiren N. Bhalani

Anaerobic Digestion for Climate Change Mitigation: A Review

• Ashwani Kumar, Ashu Rani, Mamta Choudhary

Mitigation of Microbially Influenced Corrosion of Concrete Sewers Using Nitrite

• Xuan Li, Guangming Jiang

Metabolic Engineering and Synthetic and Semi-Synthetic Pathways: Biofuel Production for Climate Change Mitigation

• Ashwani Kumar, Chamindri Witharana, Sudipti Arora, Sonika Saxena, Yuan-Yeu Yau

Handmade Paper Industry: A Green and Sustainable Enterprise and Its Challenges

• Nisha, Raman Singh, Kuldeep Singh

Bioremediation Approaches and the Role of Microbes in the Bio-sustainable Rehabilitation of Polluted Sites

• Ishani Joardar, Subhasish Dutta

Recent Bioremediation Techniques for the Removal of Industrial Wastes

• Surbhi Sinha

Pesticides: Indian Scenario on Environmental Concerns and Future Alternatives

• Preeti Mishra, Vidya Patni
• Phytoremediation

Phytoremediation: A Sustainable Solution to Combat Pollution

• Kamakshi Saxena, Touseef Hussain, Raunak Dhanker, Parool Jain, Shubham Goyal

Phytoremediation and Therapeutic Potential of Neglected Plants: An Invasive Aquatic Weeds and Ornamental Plant

• Astha Gupta, Tulika Tyagi

Phytoremediation of Coastal Saline Vertisols of Gujarat Through Biosaline Agriculture

• G. Gururaja Rao, Jitendra Chikara

Emerging Biotechnologies in Agriculture for Efficient Farming and Global Food Production

• Bablesh Ranawat, Freny Shah, Sonam Dubey, Aneesha Singh, Sandhya Mishra

Role of Beneficial Microbes in Alleviating Stresses in Plants

• Ambreen Ashar, Moazam Ali, Zeeshan Ahmad Bhutta, Ayesha Kanwal, Ujala Mehtab, Muhammad Fakhar-e-Alam Kulyar et al.

Mainstreaming of Underutilized Oilseed Safflower Crop Through Biotechnological Approaches for Improving Economic and Environmental Sustainability

• Astha Gupta

Clean Energy for Environmental Protection: An Outlook Toward Phytoremediation

• P. P. Sameena, Jos T. Puthur

This edited book focuses on the application and implementation of bioremediation and other strategies to create a sustainable and healthy environment. It provides a collection of approaches to environmental biotechnology for wastewater treatment, removal of soil heavy metals, degradation of pesticides, removal of dyes, waste management, and microbial conversion of environmental pollutants. This book brings to the fore contributions of certain globally important environmental biotechnologist. Bioremediation is a popular branch of biotechnology that involves the use of living organisms such as microorganisms (microbial remediation), bacteria, fungus (mycoremediation), and plants (phytoremediation) to bind, extract, and clean up contaminants, pollutants, and toxins from soil, groundwater, and other environments. This book is of interest to researchers, scientists, and academic faculty in environmental sciences. Also, it serves as additional reading and reference material for undergraduate and graduate students as well as postdocs in environmental, agriculture, ecology, and soil sciences. National and International policy makers will also find valuable information from this book. 

• Bioremediation
• Methylation
• Heavy Metals
• Wastewater Treatment
• Bioaccumulation
• Bioabsorption

Sudipti Arora

Ashwani Kumar

Shinjiro Ogita

Yuan -Yeu Yau

Dr. Sudipti Arora  is an Environmental Research Scientist at Dr. B. Lal Institute of Biotechnology, Jaipur, with a specialization in wastewater treatment by Vermifiltration technology and other nature-based sanitation solutions. She is also the founder of “Prakrit: a centre of excellence in Environmental Biotechnology”. Prakrit aims to provide transformative education and innovative solutions to drive the planet towards sustainability. Prakrit is dedicated to solving the problems pertaining to the environment, particularly water, wastewater, sanitation, health and hygiene, solid waste management, energy and climate change, soil and land use, good health and well-being fulfilling sustainable development goals. She has obtained her Ph.D. from the Indian Institute of Technology (IIT), Roorkee, and Master’s in Environmental Engineering from Malaviya National Institute of Technology, Jaipur. She is the Assistant Director at Dr. B. Lal Institute of Biotechnology, Jaipur, with teaching and research experience of more than 10 years with various research publications of international repute. She has also been the guest faculty at Malaviya National Institute of Technology, Jaipur. She has been working on indigenous wastewater and faecal sludge treatment through Vermifiltration technology for 8 years and has expertise in integrated solid waste management through circular economy and wastewater-based epidemiology, particularly pathogens in wastewater and AMR. She is a member of the International Water Association and has been involved in WASH projects since 2010. She has been involved in SARS-CoV-2 wastewater-based xxv epidemiology research from India with other Indian and UK International partners through global challenge research funds (GCRF 2020). She is also involved in an Indo-European project titled “Identifying best available technologies for decentralized wastewater treatment and resource recovery for India (Saraswati 2.0)” funded by DST, GOI, and “Optimization of the Disinfection Process for Wastewater Treatment by Hybrid Disinfection” funded by DST, in collaboration with MNIT, Jaipur. She is also involved in an integrated technology intervention project, funded by DST, GOI, on “transforming Aandhi village in Rajasthan towards a zero waste model through green technology interventions”. She is a guest editor and reviewer of various international journals, to name a few, She has authored and co-authored important discoveries in Environmental Biotechnology in more than 20 Science Citation Index Expanded (SCIE) journals. She has guided the research work of 50 UG and PG students. She has been honoured by various awards including “Research Excellence Award 2020 by INSc for outstanding research work on vermifiltration technology, Dainik Bhaskar “Green Parrot Award” in 2018 for her contribution to Indigenous water treatment, Best Poster Award at IWA conference on Global Challenges: Sustainable Wastewater Treatment and Resource Recovery in Kathmandu in 2014, and Young Scientist Award at 8th Uttarakhand State Science and Technology Congress in Dehradun in 2013. She is also been invited to give Expert Lectures at a number of national and international venues, conferences, and workshops

Dr. Ashwani Kumar was born (1946) to Mr. Swami Dayal Tewari and Mrs. Shanti Devi Tewari in Bandikui, Rajasthan, India. He received his B.Sc. at Agra University and his M.Sc. (Botany) at the University of Rajasthan. He was awarded a gold medal for standing first in the order of merit. His Ph.D. (1971) was under the supervision of Professor H.C. Arya and postdoctoral with Professor Dr. K-H. Neumann, and later on with Professor Dr. Sven Schubert at Justus Liebig Universitat, Giessen, Germany, as Alexander von xxvi Editors and Contributors Humboldt Fellow. His botanist father Professor Swami Dayal Tewari (M.Sc. in Botany) was his first teacher. Professor Ashwani Kumar was also selected for Indian Administrative Services (IAS: IPS) (1972) but he opted a career in botany, which being his family subject. His wife Mrs Vijay R. Kumar has also been a Professor of Botany at the University of Rajasthan in her own stead. He was appointed as Asst. Professor in 1969, Associate Professor in 1985 and Full Professor from 1996 to 2007. Then he was an Adjunct Professor until 2016. He along with members of COC introduced Integrated Biotechnology, a 5-year course in Rajasthan. In recognition of his research contributions, Dr. Kumar was awarded the Alexander von Humboldt Fellowship for 1977–1979, with several resumption of fellowships until 2017, British Council Visitorship, UK (1986); Visiting Professorship at Toyama Medical and Pharmaceutical University in Japan (1999–2000); Toyama Prefectural University, Japan (2011); and INSA-DFG visiting Professorship in Germany (1997). He holds a diploma in German language and has a certificate in French language. His area of research includes photosynthesis in vitro and in vivo, biotic and abiotic resistance, ethno botany, bioenergy and presently understanding salinity resistance in maize. He has also carried out research projects granted by UGC, USDA-ICAR, MNES, CSIR, DST, DBT and FACT. He attended a large number of national and international conferences as an invited speaker and served as chair or co-chair of the International Botanical Conference, Berlin, and EU Biomass conferences. He has published 220 research papers in national and international journals and 23 books, of which 10 books are authored and 13 are edited from reputed publishers such as Springer and IK. He is a member of the editorial board of Current Trends in Biotechnology and Pharmacy. He has guided 39 research students to Ph.D. at the University of Rajasthan, Jaipur, India. He is an elected Fellow of Botanical Society, Fellow of Phytopathological Society, Fellow of the Indian Society of Mycology and Plant Pathology, Fellow of Mendelian Association, Fellow of Association of Biotechnology and Pharmacology and Fellow of Indian Fern Society. He received V. Puri Medal as Botanist and Teacher’s Excellence Editors and Contributors xxvii award of CEE in 2015. He has been a consultant in a World Bank Project sanctioned to SPRI-HPPI, President of Commonwealth Human Ecology Council (India chapter) and presently President of Indian Botanical Society and President of the Society for Promotion of Plant Science 2021–2022.

Dr. Shinjiro Ogita has over two decades of experience in the field of plant biotechnology. In 1992, he started his research career as a master’s student at the United Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Japan, and in 1997, he received his Ph.D. in agriculture (subject: sciences of resources and environment). He is an expert in the field of cell and tissue culture and transformation technologies for higher plants. He has worked at the following institutes based on projects worked: embryogenic capacity of elite coniferous trees at the Laboratory of Cell Manipulation, Division of Bio-resources Technology, Forestry and Forest Products Research Institute, Ministry of Agriculture, Forestry and Fisheries, Japan (October 1997–September 2000). • To establish genetically modified decaffeinated coffee plants at the Laboratory of Plant Molecular Breeding, Research and Education Centre for Genetic Information, Nara Institute of Science and Technology (NAIST), Japan (October 2000–March 2003). • To teach plant biotechnology, microbiology and molecular biology as assistant professor (2003–2006), lecturer (2006–2010) and associate professor (2010–2015) at the Laboratory of Plant and Cell Engineering, Biotechnology Research Centre and Department of Biotechnology, Toyama Prefectural University (TPU), Japan (April 2003–March 2015). He presently works as a full professor at the Faculty of Life and Environmental Sciences, Department of Life Sciences, Prefectural University of Hiroshima (PUH), Japan (April 2015).

Dr. Yuan -Yeu Yau obtained his master’s and Ph.D. from the University of Wisconsin-Madison, USA. He worked as a postdoc and a specialist at the University of California-Berkeley and Plant Gene Expression Centre (USDA-ARS) in Albany, California, working in the areas of plant biotechnology, plant breeding, plant biochemistry and plant physiology. Dr. Yau worked on projects with grants supported by the NFS (National Science Foundation), NIH (National Institutes of Health), USDA, Cotton Incorporated, California Fresh Carrot Advisory Board and Northeastern State University. These projects include carrot breeding for fresh market, cottonseed gossypol (a toxic compound) removal and the development of clean-gene technology and of stroke drug using molecular farming. Dr. Yau joined Dr. David W. Ow’s team (UC-Berkeley) in developing an operation system for precision transgene integration, stacking (at same locus) and deletion (e.g. removal of SMG) using microbial site-specific recombination (SSR) systems. Professor Yau has authored and co-authored important discoveries in several Science Citation Index (SCI) and Science Citation Index Expanded (SCIE) journals. Dr. Yau also serves as a reviewer for several international scientific journals. He also served as a reviewer for several book proposals for Springer Nature. He is also an editor of several Springer Nature books. He joined the Chinese Academy of Sciences as a professor in 2010 and then joined Northeastern State University of Oklahoma (USA) as a research scientist and adjunct professor in 2012. Dr. Yau in an active member of Research Gate. He mentors numerous students from all over the world on Research Gate.

Book Title : Biotechnological Innovations for Environmental Bioremediation

Editors : Sudipti Arora, Ashwani Kumar, Shinjiro Ogita, Yuan -Yeu Yau

DOI : https://doi.org/10.1007/978-981-16-9001-3

Publisher : Springer Singapore

eBook Packages : Biomedical and Life Sciences , Biomedical and Life Sciences (R0)

Copyright Information : The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2022

Hardcover ISBN : 978-981-16-9000-6 Published: 05 August 2022

Softcover ISBN : 978-981-16-9003-7 Published: 05 August 2023

eBook ISBN : 978-981-16-9001-3 Published: 03 August 2022

Edition Number : 1

Number of Pages : XXXV, 1063

Number of Illustrations : 1 b/w illustrations

Topics : Biotechnology , Environment, general , Environmental Engineering/Biotechnology

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Futuristic Plant Microbes Biotechnology and Bioengineering

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Plant-microbe interactions have long been recognized as crucial determinants of plant health, growth, and productivity. Recent breakthroughs in molecular biology, genomics, and bioinformatics have unveiled the intricate mechanisms underlying these interactions, sparking a revolution in biotechnological and ...

Keywords : plant microbes, synthetic biology, plant-microbe interactions, metabolic engineering, biocontrol agents, plant disease, microbiome engineering, microbial biotransformations, sustainable agriculture, bioremediation, phytoremediation, nanotechnology

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Our team forms partnerships with these microbial communities. This means managing the near-limitless metabolic diversity of the microorganisms while using their services to clean up pollution, produce renewable resources and improve our health. In return, we provide the microorganisms with a good life as they carry out their natural metabolism. It’s a win-win solution. 

We apply the most advanced tools of molecular microbial ecology, chemistry, microscopy and mathematical modeling to think like the microorganisms and, in turn, create systems that allow the microorganisms to provide beneficial services, ranging from sustainable environmental processes and nutrient and energy recovery to making humans healthier. 

Our culture begins with our diverse set of researchers who come from many disciplines within engineering, life sciences, chemistry and more. Partnerships are common within our center, as well as with other groups in ASU, national and international universities and practitioners. 

To date, our center has spun out two Biodesign research centers: 

• Biodesign Center for Environmental Health Engineering
• Biodesign Center for Health Through Microbiomes

Managing microbial communities for societal service is achieved through cross-disciplinary and team-based research in the areas of engineering, sciences, sustainability and biological design.  

Major efforts of our center, include:  

• Mathematical modeling specific to environmental biotechnology. Lab research ranges from fundamental concepts like chemical speciation, biofilms and microbial ecology, to practical applications including the microbial electrochemical cells, wastewater treatment, bioremediation and membrane biofilm reactors. This work is supported by the Rittmann Lab.
• Production of energy or high-value chemicals through microbiological technologies. We make use of microorganisms and their complex enzymatic machinery to carry out reactions that are difficult or impossible through any other known chemical route. Our main research interests are microbial electrochemistry, fermentations and photosynthetic biofuel production. This work is supported by the Rittmann Lab.
• Water sustainability. We minimize the production of waste byproducts and advance the water–energy–food nexus. Our water efforts include drinking water and wastewater treatment, natural aquatic systems, treatment of water at various lifecycle stages, water conservation, valuable material recovery and sequestration of harmful contaminants. This work is supported by the Rittmann Lab.
• Research on microbes. We study the ecology of carbon-rich ecosystems, the interactions and activities of microbes as potential ecosystem drivers, and the genomics and evolution of microbes to track their mechanisms of change. We also focus on novel groups of methane-producing Archaea and interacting bacteria in anaerobic, high-carbon content of natural or human-engineered environments. This work is supported by the Cadillo Lab.
• Systems approach to water quality and treatment . We consider global drivers such as urbanization, climate change, biogeochemical cycles, sustainable engineering and disruptive innovation. 
• Field applications. We work in biological, chemical and physical characterization of soils, biochemical reactive transport and multiphase flow in porous media. Our team works with the ASU Center for Biomediated and Bioinspired Geotechnics to develop and transfer technologies from a proof-of-concept in the laboratory to field scale demonstration projects for a variety of geotechnical and geoenvironmental engineering applications. This work is supported by the Delgado Lab and the Torres Lab.
• Nanomaterials. Our team produces and uses nanomaterials in concert with microorganisms. We work with the NSF Center for Nanoenabled Water Treatment to develop novel nanobiotechnologies for detoxifying recalcitrant pollutants to purify a wide range of waters and wastewaters. This work is supported by the Rittmann Lab.
• Circular economy. We recover economic value of components of wastes. By applying membrane-based techniques, these valuable components are advanced as high-value products to achieve more cost- and energy-effective products. This circular economy tackles environmental challenges, such as greenhouse gases mitigation, while also generating economic benefits. The work, led by Lai and Rittmann, is supported by Global Center for Water Technology, the center for Science and Technology for Enhancing Phosphorus Sustainability and private industry collaboration.

Areas of investigation

• Advanced oxidation and biodegradation (Rittmann, Delgado and Zhou) in the Rittmann Lab and the Delgado Lab.
• Amazon peatlands: C and N stocks and cycling in the Cadillo Lab.
• Bio-based ground improvement through microbially induced carbonate precipitation in the Rittmann Lab.
• Biofilms (Zhou, Rittmann and Krajmalnik-Brown) in the Rittmann Lab and the Center for Health Through Microbiomes . 
• Biofilm drinking water treatment (Zhou and Rittmann) in the Rittmann Lab.
• Bio-mediated iron precipitation for permeability reduction in the Torres Lab and the Rittmann Lab.
• Bioremediation in all labs — see the Rittmann Lab for information.
• Coal methanogenesis: energy production and water management in the Cadillo Lab.
• Cultural diversity of bacteria and archaea in the Cadillo Lab and the Center for Health Through Microbiomes . 
• Fermentations in the Torres Lab, Delgado Lab and Rittmann Lab. 
• Genomic adaptations and physiology of methanogens in the Cadillo Lab.
• Liquefaction mitigation through desaturation by stimulating nitrate-reducing bacteria to produce nitrogen gas in the Rittmann Lab. 
• Mathematical modeling in the Rittmann Lab.
• Membrane biofilm reactors (Zhou and Rittmann) in the Rittmann Lab.
• Methanogenesis and anaerobic digestion (Rittmann, Delgado and Krajmalnik-Brown) in the Delgado Lab, the Rittmann Lab and the Center for Health Through Microbiomes . 
• Microalgae technology in the Rittmann Lab.
• Microbial ecology (in all labs).
• Microbial electrochemistry in the Torres Lab and Rittmann Lab.
• Microbial products in the Rittmann Lab.
• Nutrient recovery in the Rittmann Lab.
• Photobioenergy in the Torres Lab and Rittmann Lab.
• Northern peatlands: Climate and its effects on microbial activity in the Cadillo Lab.
• Novel biotechnologies (in all labs).
• Resource recovery (Zhou and Rittmann) in the Rittmann Lab. 
• Wastewater treatment (Zhou and Rittmann) in the Rittman Lab. 

Capabilities

• Extensive chemical analytical facility.  
• Fully equipped microbiology and molecular biology facility for research on classical microbiology and molecular microbial ecology. 
• Custom-designed process laboratory that maximizes flexibility for supporting bench-scale reactor systems.  
• Photobioenergy research laboratory with photobioreactor facility.  
• Deep expertise and capacity in molecular microbial ecology, mathematical modeling and novel biotechnologies.  
• Expertise in the biological, chemical, and physical characterization of soils, biochemical reactive transport and multiphase flow in porous media and affiliate with the Center for Biomediated and Bioinspired Geotechnics.

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Managing microbial communities that provide services to make society more sustainable.

Research Topics & Ideas

Biotechnology and Genetic Engineering

If you’re just starting out exploring biotechnology-related topics for your dissertation, thesis or research project, you’ve come to the right place. In this post, we’ll help kickstart your research topic ideation process by providing a hearty list of research topics and ideas , including examples from recent studies.

PS – This is just the start…

We know it’s exciting to run through a list of research topics, but please keep in mind that this list is just a starting point . To develop a suitable research topic, you’ll need to identify a clear and convincing research gap , and a viable plan  to fill that gap.

If this sounds foreign to you, check out our free research topic webinar that explores how to find and refine a high-quality research topic, from scratch. Alternatively, if you’d like hands-on help, consider our 1-on-1 coaching service .

Biotechnology Research Topic Ideas

Below you’ll find a list of biotech and genetic engineering-related research topics ideas. These are intentionally broad and generic , so keep in mind that you will need to refine them a little. Nevertheless, they should inspire some ideas for your project.

• Developing CRISPR-Cas9 gene editing techniques for treating inherited blood disorders.
• The use of biotechnology in developing drought-resistant crop varieties.
• The role of genetic engineering in enhancing biofuel production efficiency.
• Investigating the potential of stem cell therapy in regenerative medicine for spinal cord injuries.
• Developing gene therapy approaches for the treatment of rare genetic diseases.
• The application of biotechnology in creating biodegradable plastics from plant materials.
• The use of gene editing to enhance nutritional content in staple crops.
• Investigating the potential of microbiome engineering in treating gastrointestinal diseases.
• The role of genetic engineering in vaccine development, with a focus on mRNA vaccines.
• Biotechnological approaches to combat antibiotic-resistant bacteria.
• Developing genetically engineered organisms for bioremediation of polluted environments.
• The use of gene editing to create hypoallergenic food products.
• Investigating the role of epigenetics in cancer development and therapy.
• The application of biotechnology in developing rapid diagnostic tools for infectious diseases.
• Genetic engineering for the production of synthetic spider silk for industrial use.
• Biotechnological strategies for improving animal health and productivity in agriculture.
• The use of gene editing in creating organ donor animals compatible with human transplantation.
• Developing algae-based bioreactors for carbon capture and biofuel production.
• The role of biotechnology in enhancing the shelf life and quality of fresh produce.
• Investigating the ethics and social implications of human gene editing technologies.
• The use of CRISPR technology in creating models for neurodegenerative diseases.
• Biotechnological approaches for the production of high-value pharmaceutical compounds.
• The application of genetic engineering in developing pest-resistant crops.
• Investigating the potential of gene therapy in treating autoimmune diseases.
• Developing biotechnological methods for producing environmentally friendly dyes.

Biotech & GE Research Topic Ideas (Continued)

• The use of genetic engineering in enhancing the efficiency of photosynthesis in plants.
• Biotechnological innovations in creating sustainable aquaculture practices.
• The role of biotechnology in developing non-invasive prenatal genetic testing methods.
• Genetic engineering for the development of novel enzymes for industrial applications.
• Investigating the potential of xenotransplantation in addressing organ donor shortages.
• The use of biotechnology in creating personalised cancer vaccines.
• Developing gene editing tools for combating invasive species in ecosystems.
• Biotechnological strategies for improving the nutritional quality of plant-based proteins.
• The application of genetic engineering in enhancing the production of renewable energy sources.
• Investigating the role of biotechnology in creating advanced wound care materials.
• The use of CRISPR for targeted gene activation in regenerative medicine.
• Biotechnological approaches to enhancing the sensory qualities of plant-based meat alternatives.
• Genetic engineering for improving the efficiency of water use in agriculture.
• The role of biotechnology in developing treatments for rare metabolic disorders.
• Investigating the use of gene therapy in age-related macular degeneration.
• The application of genetic engineering in developing allergen-free nuts.
• Biotechnological innovations in the production of sustainable and eco-friendly textiles.
• The use of gene editing in studying and treating sleep disorders.
• Developing biotechnological solutions for the management of plastic waste.
• The role of genetic engineering in enhancing the production of essential vitamins in crops.
• Biotechnological approaches to the treatment of chronic pain conditions.
• The use of gene therapy in treating muscular dystrophy.
• Investigating the potential of biotechnology in reversing environmental degradation.
• The application of genetic engineering in improving the shelf life of vaccines.
• Biotechnological strategies for enhancing the efficiency of mineral extraction in mining.

Recent Biotech & GE-Related Studies

While the ideas we’ve presented above are a decent starting point for finding a research topic in biotech, they are fairly generic and non-specific. So, it helps to look at actual studies in the biotech space to see how this all comes together in practice.

Below, we’ve included a selection of recent studies to help refine your thinking. These are actual studies,  so they can provide some useful insight as to what a research topic looks like in practice.

• Genetic modifications associated with sustainability aspects for sustainable developments (Sharma et al., 2022)
• Review On: Impact of Genetic Engineering in Biotic Stresses Resistance Crop Breeding (Abebe & Tafa, 2022)
• Biorisk assessment of genetic engineering — lessons learned from teaching interdisciplinary courses on responsible conduct in the life sciences (Himmel et al., 2022)
• Genetic Engineering Technologies for Improving Crop Yield and Quality (Ye et al., 2022)
• Legal Aspects of Genetically Modified Food Product Safety for Health in Indonesia (Khamdi, 2022)
• Innovative Teaching Practice and Exploration of Genetic Engineering Experiment (Jebur, 2022)
• Efficient Bacterial Genome Engineering throughout the Central Dogma Using the Dual-Selection Marker tetAOPT (Bayer et al., 2022)
• Gene engineering: its positive and negative effects (Makrushina & Klitsenko, 2022)
• Advances of genetic engineering in streptococci and enterococci (Kurushima & Tomita, 2022)
• Genetic Engineering of Immune Evasive Stem Cell-Derived Islets (Sackett et al., 2022)
• Establishment of High-Efficiency Screening System for Gene Deletion in Fusarium venenatum TB01 (Tong et al., 2022)
• Prospects of chloroplast metabolic engineering for developing nutrient-dense food crops (Tanwar et al., 2022)
• Genetic research: legal and ethical aspects (Rustambekov et al., 2023). Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts (Thagun et al., 2022)
• The role of genetic breeding in food security: A review (Sam et al., 2022). Biotechnology: use of available carbon sources on the planet to generate alternatives energy (Junior et al., 2022)
• Biotechnology and biodiversity for the sustainable development of our society (Jaime, 2023) Role Of Biotechnology in Agriculture (Shringarpure, 2022)
• Plants That Can be Used as Plant-Based Edible Vaccines; Current Situation and Recent Developments (İsmail, 2022)

As you can see, these research topics are a lot more focused than the generic topic ideas we presented earlier. So, in order for you to develop a high-quality research topic, you’ll need to get specific and laser-focused on a specific context with specific variables of interest.  In the video below, we explore some other important things you’ll need to consider when crafting your research topic.

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If you’re still unsure about how to find a quality research topic, check out our Research Topic Kickstarter service, which is the perfect starting point for developing a unique, well-justified research topic.

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Huge database gives insight into salmon patterns at sea

A massive new analysis of high seas salmon surveys is enhancing the understanding of salmon ecology, adding details about where various species congregate in the North Pacific Ocean and their different temperature tolerances.

The project, led by researchers at the University of Alaska Fairbanks, integrates numerous international salmon studies from the North Pacific dating back to the 1950s. Although many individual reports were published by nations and agencies that funded those efforts, they were never fully compiled into an overarching database or analyzed comprehensively at this scale. This new research effort builds upon the extensive and valuable past body of work on the marine component of the salmon life cycle.

Together the data represent a trove of more than 44,000 high seas survey gear hauls across the North Pacific, netting over 14 million salmon. That ocean-based data also provides a contrast from the bulk of salmon research, which tends to focus on river habitat.

"This is a portion of the salmon life cycle that arguably gets overlooked, at least in terms of the grand investment in salmon research," said Curry Cunningham, an assistant professor at UAF's College of Fisheries and Ocean Sciences. "As someone who always wondered where all these fish went when they left Bristol Bay, seeing that pattern come to life was so satisfying."

Although methods and data varied among the studies, they consistently noted the water temperature and location where salmon were caught. That data allowed the research team to create maps with unprecedented detail showing the areas that various salmon species occupy while at sea.

For example, the maps highlight a hotspot for Chinooks gathering in the Bering Sea. They chart a path that maturing sockeye tend to take from the North Pacific and Gulf of Alaska toward the Alaska Peninsula, traveling through passes into the Bering Sea outside Bristol Bay throughout the spring and summer.

"It's not as if all these salmon are going to some party in the middle of the Pacific," said lead author Joe Langan, who worked on the project as a postdoctoral fellow at UAF. "This gives us a broad look at where they go."

The study also produced more evidence that cold-water tolerance varies significantly among salmon species. Sockeye and chum salmon were commonly found in temperatures down to just a few degrees above freezing, while coho and steelhead were absent from the coldest areas. Chinook and pink salmon lived in the middle of those ranges.

Those tendencies are likely to influence which species will be most capable of shifting to colder waters as their traditional ranges are warmed by climate change, Cunningham said.

The heart of the dataset is from the 1950s to the 1990s, and the research required digging up forgotten reports from varying sources. Skip McKinnell, a retired Department of Fisheries and Oceans Canada researcher and expert in high seas salmon research, participated in some of the earlier surveys. He helped dredge up, compile and standardize many of those older reports.

"It was more of a search and rescue mission rather than convincing anyone to provide their data," Langan said.

The project was funded by the National Oceanic and Atmospheric Administration's Office of Law Enforcement, which hopes to use information about where salmon are concentrated at sea to help curb illegal fishing. The centralized database will be housed by the North Pacific Anadromous Fish Commission and made publicly available.

• Marine Biology
• Environmental Awareness
• Oceanography
• Global Warming
• Coho salmon
• Atlantic salmon
• Short-tailed Albatross
• Fish migration
• El Niño-Southern Oscillation

Story Source:

Materials provided by University of Alaska Fairbanks . Note: Content may be edited for style and length.

Journal Reference :

• Joseph A. Langan, Curry J. Cunningham, Jordan T. Watson, Skip McKinnell. Opening the black box: New insights into the role of temperature in the marine distributions of Pacific salmon . Fish and Fisheries , 2024; DOI: 10.1111/faf.12825

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Biotechnology Research Topics

What is biotechnology.

What first pops up in your mind when you hear the term Biotechnology? Maybe you started thinking of GMOs ( Genetically Modified Organisms ), transgenic cloning, and other gene therapies. Of course, you got it right, but the horizon of biotechnology is not so tiny. It has a wide range of applications in the industry that can improve our living standards. Let us first understand the term Biotechnology. In simple words, it is the utilization of living organisms or their components in the industrial sector to generate various products that are beneficial for the human race. We have been utilizing microorganisms for more than thousands of years to develop useful commodities such as cheese, bread, and various other dairy-related products. Even its implementation in the medical sector has led to the manufacturing of different vaccines, biofuel, chitosan-coated dressing for wounds, brewing, and even age-defying products. As the biotechnology scope is expanding day by day, researchers felt an urge to classify main areas and types of biotechnology depending on some commonalities and their ultimate objectives:

Red Biotechnology- involves the utilization of organisms for upgrading the quality of health care departments and aiding the body’s immune system to fight against various diseases. Examples include; the development of different vaccines, antibiotics, medicinal drugs, and various molecular techniques.

White Biotechnology- mainly comprises industrial biotechnology and involves the utilization of microorganisms and their by-products for manufacturing more eco-friendly and energy-efficient products. White biotechnology examples include the production of biofuel, Lactic acid, and 3- hydroxy propionic acid.

Yellow Biotechnology- it is related to the use of Biotech in the food production area, i.e., making bread, cheese, beer, and wine by the fermentation process.

Grey Biotechnology – mainly deals with the removal of pollutants from the environment by using various microorganisms and plants. For example., different strains of bacteria can be used for the degradation of kitchen waste into compost.

  Green Biotechnology- concentrates on the agriculture sector and focuses on generating new varieties of plants and producing good quality bio-pesticides & bio-fertilizers.

  Blue Biotechnology – it mainly refers to the utilization of aquatic or marine organisms to create goods that can aid various industrial processes, such as using Chitosan (sugar derived from the shells of crabs and shrimps) for the dressing of wounds.

Biotechnology Topics for Research Paper

In the modern world, students are apprehending the benefits of Biotech and want to study it with more enthusiasm and interest. They are actively opting for this subject and compiling their research work to contribute their efforts in the field of Biotechnology. They are indulged in exhaustive research to find the best topic for the research purpose. So, here are a few potential research topics in the domain of Biotechnology:

Red Biotechnology Research Topics:

• Studying the relationship between the intake of iron-folic acid during pregnancy and its impact on the overall health of the fetus.
• Pharmacogenomics of antimicrobial drugs.
• Identifying the biomarkers linked with breast cancer.
• Study the medicinal value of natural antioxidants.
• Study the structure of coronavirus spike proteins.
• Studying the immune response of stem cell therapy.
• Utilization of CRISPR-Cas9 technology for genome editing.
• Application of Chitosan in tissue engineering and drug delivery.
• Study the therapeutic effects of cancer vaccines.
• Utilizing PacBio sequencing for the genome assembly of model organisms.
• Study the relationship between the suppression of mRNA and its effect on stem cell expansion.
• Study the application of nanoprobes in molecular imaging.
• Incorporating biomimicry for the detection of tumor cells.
• Study of immune-based therapies in treating COVID-19.
• Regulation of immune response using the cellular and molecular mechanism
• Microchip implantation – a vaccine for coronavirus.
• The Use of CRISPR for Human Genome Editing

Yellow Biotechnology Research Topics:

• Production of hypoallergenic milk.
• Production of hypoallergenic fermented foods.
• Yellow enzymes subclassification and their characterization.

White Biotechnology Research Topics:

• Bioconversion of cellulose to yield industrially important products.
• Studying the inhibitors of endocellulase and exocellulase.
• Fungal enzymes used in the production of chemical glue.
• Mechanism of fungal enzymes in the biodegradation of lignin.
• Studying gut microbiota in model organisms.
• Study the lactic acid bacteria for probiotic potential.
• Purification of thermostable phytase.
• Mesophilic and Thermophilic aerobic and anaerobic bacteria from compost.
• Study the dietary strategies for the prophylaxis of Alzheimer’s and dementia.
• Examine the positive effects of probiotics and prebiotics on the nervous system.

Examples of Grey Biotechnology Research Topics:

• Production of sustainable, low-cost, and environmentally friendly microbial biocement and biogrouts.
• Use of microorganisms for the recovery of shale gas.
• Studying the procedure of natural decomposition.
• Treatment of grey water in a multilayer reactor with passive aeration.
• Excavation of various anaerobic microbes using grey biotechnology.
• Improving the biodegradation of micro-plastics using GMOs.
• Removal of pollutants from the land.
• Use of microbes to excavate the hidden metals from earth.
• Managing the processes of environmental biotechnology using microbial ecology.
• In situ product removal techniques using the process of biocatalysis.
• Production of biodegradable, disposable plastic for the storage of food.
• Plastic waste decomposition management.  
• Maintaining a healthy equilibrium between biotic and abiotic factors using biotechnological tools.
• Recycling of biowastes.
• Restoration of biodiversity using tools.
• Improved Recombinant DNA technology for bioremediation.
• Gold biosorption using cyanobacterium.
• Improved bioremediation of oil spills.
• Biodegradation of oil and natural gas.

Blue Biotechnology Research Ideas:

• Various bioactive compounds derived from marine sponges.
• Controlling the emerged biological contaminant using the sustainable future.
• Protecting the environment using grey, blue, and green biotechnology.  
• Exploring marine biota which survives the extreme conditions.
• Studying the patterns of Arctic and Antarctic microbiota for the benefits of humans.
• Excavation of bioactive molecules from extreme environmental conditions.
• Studying the potential of sponge-associated microbes.
• Mercury labeling in the fish using markers.
• Sea urchin repelling ocean macroalgal afforestation.
• Microbial detection techniques to find sea animals.
• Studying the mechanisms in deep-sea hydrothermal vent bacteria.
• Production of antibiotics using marine fungi.
• Exploring the biotechnological potential of Jellyfish associated microbiome.  
• Exploring the potential of marine fungi in degrading plastics and polymers.
• Expl oring the biotechnological potential of dinoflagellates.

Green Biotechnology Research Paper Topics:

• Detection of endosulfan residues using biotechnology in agricultural products.
• Development of ELISA technique for the detection of crops’ viruses.
• Use of Green Fluorescent Protein (GFP) as a cytoplasmic folding reporter.
• E.coli as an all-rounder in biotechnological studies.
• Improving the water quality for drinking using E.coli consortium.
• E.coli characterization isolated from the zoo animals’ feces.  
• Biocatalysis and agricultural biotechnology in situ studies.
• Improving the insect resistance of the crops.
• Improving the nutritional value and longer shelf life of GM crops.
• Improving the qualities of hydroponic GM plants.
• Reducing the cost of agriculture using bio-tools.
• Production of heavy cotton balls in agricultural biotechnology using in situ technique.
• Steps to minimize soil erosion using the tools of biotechnology.
• Enhancement of vitamin levels in GM Foods .
• Improving pesticide delivery using biotechnology.
• Comparison of folate biofortification of different crops.
• Photovoltaic-based production of crops in the ocean.
• Application of nanotechnology in the agricultural sector.
• Study the water stress tolerance mechanisms in model plants.

Combination and Analytical Topics:

• Sequencing of infectious microbes using molecular probes.
• Production and testing of human immune boosters in experimental organisms.
• Comparative genomic analysis using the tools of bioinformatics.
• Arabinogalactan protein sequencing using computational methods.
• Comparative analysis of different protein purification techniques.
• Oligonucleotide microarrays used in the diagnosis of the microbes.
• Uses of different techniques in biomedical research including microarray technology.
• Microbial consortium used to produce the greenhouse effect.
• Computational analysis of different proteins obtained from marine microbiota.
• Gene mapping of E.coli using different microbial tools.
• Computational analysis and characterization of the crystallized proteins in nature.
• Improving the strains of cyanobacterium using gene sequencing.
• mTERF protein used to terminate the mitochondrial DNA transcription in algae.  
• Reverse phase column chromatography used to separate proteins.
• Study of different proteins present in Mycobacterium leprae.
• Study the strategies best suitable for cloning RNA
• Study the application of nanocarriers for the gene expression in model plants.
• Exploring thermotolerant microorganisms for their biotechnological potential.

Biotechnology is full of research prospects. Various research and development companies are working day and night to achieve the required outcomes for different branches of biotechnology. If you find these list of Biotechnology research topics helpful, you may visit our blog for further assistance.

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Camera rig used for capturing images of oyster reefs

Restored oyster reef in Harris Creek, Maryland

Harvested oyster reef in Broad Creek, Maryland

About the project.

Oysters serve an important role in the economy and ecology of the Chesapeake Bay region, but historical overfishing and disease have resulted in a large decline in their abundance. Oyster restoration is ongoing throughout the Chesapeake Bay in order to replenish oyster populations. Monitoring of the oyster reefs that have undergone restoration is necessary to assess the success of the efforts.

Researchers at SERC are partnering with local riverkeeper organizations to capture photographs of restored oyster reefs in order to assess the structure of oyster reefs and the associated organisms living on the reefs. Collected images will be made publicly available online for analysis by members of the public.

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Biotechnology articles from across Nature Portfolio

Biotechnology is a broad discipline in which biological processes, organisms, cells or cellular components are exploited to develop new technologies. New tools and products developed by biotechnologists are useful in research, agriculture, industry and the clinic.

Reducing greenhouse gas emissions with nanofertilizers

A growing population, climate change and the environmental impacts of conventional agricultural practices are worsening food insecurity. New research shows that use of nanofertilizers can increase food production while simultaneously decreasing the negative environmental effects of agriculture.

• Christian O. Dimkpa
• Christy L. Haynes
• Jason C. White

Co-evolved genes improve the biosynthesis of secondary metabolites

A new engineering strategy for improving the biosynthesis of secondary metabolites in Streptomyces has been developed through the analysis of genes co-evolved with biosynthetic gene clusters. This strategy has been verified in 11 Streptomyces strains to enhance production of 16,385 metabolites, showing potential applications in drug discovery and industrial production.

Genetically encoding colors and images into bioengineered microbial materials

Using synthetic biology, we engineered a cellulose-producing bacterium that can produce eumelanin and respond to light, so that it is possible to grow a microbial leather material that is colored black or contains projected black patterns.

Related Subjects

• Animal biotechnology
• Applied immunology
• Assay systems
• Biomaterials
• Biomimetics
• Cell delivery
• Environmental biotechnology
• Expression systems
• Functional genomics
• Gene delivery
• Gene therapy
• Industrial microbiology
• Metabolic engineering
• Metabolomics
• Molecular engineering
• Nanobiotechnology
• Nucleic-acid therapeutics
• Oligo delivery
• Peptide delivery
• Plant biotechnology
• Protein delivery
• Regenerative medicine
• Stem-cell biotechnology
• Tissue engineering

Latest Research and Reviews

Local environment in biomolecular condensates modulates enzymatic activity across length scales

Here, the authors show that biomolecular condensates can enhance enzymatic rates by creating distinct solvent environments compared to the surrounding solution, and this emergent property can manifest within assemblies as small as nanometers.

• Marcos Gil-Garcia
• Ana I. Benítez-Mateos
• Paolo Arosio

Engineering a synthetic gene circuit for high-performance inducible expression in mammalian systems

Inducible gene expression systems can be used to control the expression of a gene of interest by means of small molecules. Here the authors present CASwitch, a synthetic gene circuit platform enhancing inducible gene expression systems by reducing leakiness and boosting fold induction, for real world applications like gene therapy vector production and biosensors.

• Giuliano De Carluccio
• Virginia Fusco
• Diego di Bernardo

Multimodal nanoimmunotherapy engages neutrophils to eliminate Staphylococcus aureus infections

Antimicrobial resistance is becoming more prevalent. Here the authors use multimodal nanoparticles to modulate the infected microenvironment, recruit neutrophils and alleviate hypoxia to restore neutrophil function, demonstrating therapeutic efficacy against MRSA infections in mice.

• Jingcheng Zhu
• Shaoqin Gong

A multi-cancer early detection blood test using machine learning detects early-stage cancers lacking USPSTF-recommended screening

• Janet Vittone
• Jordan J. Karlitz

Riboflavin based setup as an alternative method for a preliminary screening of face mask filtration efficiency

• Aida Cavallo
• Tamer Al Kayal
• Lorena Tedeschi

The buzz about honey-based biosurveys

• Paton Vuong
• Anna Poppy Griffiths
• Parwinder Kaur

News and Comment

Mapping the global landscape for induced pluripotent stem cells from patents and clinical trials

Although the clinical application of induced pluripotent stem cells is still in its infancy, the development of iPSC technologies reflected by an increasing number of patents provides hope that they will realize their therapeutic potential.

Safe delivery of ICI therapy through sub-urothelial injection

• Maria Chiara Masone

SIMPLE-seq to decode DNA methylation dynamics in single cells

In this Tools of the Trade article, Dongsheng Bai and Chenxu Zhu describe SIMPLE-seq, a scalable single-cell sequencing method that simultaneously decodes the cytosine modifications 5mC and 5hmC.

• Dongsheng Bai

Response to the Commentary on: Safety and feasibility of percutaneous needle tunneling with platelet-rich plasma injections for Peyronie’s disease in the outpatient setting: a pilot study

• Ahmed S. Zugail
• Muaath Alshuaibi
• Sébastien Beley

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